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The osmotic pressure is a colligative property and mathematically can be represented as p = (nRT /V ) i, where o is the osmotic pressure in atmospheres; n is the number of moles of solute; R is the ideal gas constant 00821 L atm/Kmol; T is the Kelvin temperature; V is the volume of the solution; and i is the van t Hoff factor Measurements of the osmotic pressure can be used to calculate the molar mass of a solute This is especially useful in determining the molar mass of large molecules such as proteins For example, a solution prepared by dissolving 895 mg of a gene fragment in 350 mL of water has an osmotic pressure of 0335 torr at 250 C Assuming the fragment is a nonelectrolyte, determine the molar mass of the gene fragment Rearrange p = (nRT/V ) i to n = p V/RT (i = 1 for a nonelectrolyte)

Substance B is secreted by the tubules PAH; creatinine; inulin; urea; sodium; glucose The answer is 001 The excreted amount of sodium is as follows: 75 mmol/L 3 2 mL/min 3 0001 L/mL 5 015 mmol/min Because sodium is freely filtered, the filtered amount is the GFR 3 P Na, and GFR is the same as CIn GFR 5 CIn 5 50 mg/L 4 1 mg/L 3 2 mL/min 5 100 ml/min, or 01 L/min Filtered sodium 5 150 mmol/L 3 01 L/min 5 15 mmol/min Therefore, the fraction of the filtered load that is excreted, fractional excretion, is 015 mmol/min 4 15 mmol/min 5 001 The answer is A With only one-fifth of the excretory capacity remaining, the plasma concentration will rise by a factor of 5 to achieve the same excretion

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(0335 torr )(350 mL) 1 atm 1 L = 6 30 1 0 1 L atm 760 torr 1000 mL 0 0821 ( 298 2 K ) mol K

Instead of being able to transmit 20 compressed streams in place of 1 pulse code modulation (PCM) stream, we can now only transmit 7 If several voice frames are packed into one RTP packet, transmission efficiency will be improved dramatically This will, however, cause an increase in delay because the RTP packet cannot be transmitted until its entire payload has been assembled If multiple VoIP streams are being sent from one gateway to another, it is possible to put frames from several different streams into the same RTP packet This brings the advantages of improved transmission efficiency with a minimal increase in delay and only a small increase in complexity Within a dedicated VoIP network, much of the information carried in the RTP/UDP/IP header is redundant; hence, it is possible to compress this The degree to which this is possible will be limited because at least the IP header is required to route packets through the network unless MPLS or some similar tag or label-switching protocol is used MPLS maps an IP address onto a label that can be used by label-switched routers (LSRs) to efficiently transport network traffic This approach has the advantage that traffic can be aggregated into streams with common QoS

4 1 The answer is false Flux by a uniporter is always passive, down the electrochemical gradient Flux by a symporter may be active depending on direction Flux via an ATPase is always active (Theoretically, it could pump downhill, but this does not normally occur) Most regions of the nephron have tight junctions that are far less leaky than those found in the proximal tubule, and most apical membranes are far less permeable to water As a result, it is possible to sustain much larger osmotic gradients across the epithelium in tubular regions beyond the proximal tubule Reabsorption beyond the proximal tubule is generally not iso-osmotic The answer is true The tubular epithelium is quite permeable to many (but not all small solutes), but the peritubular endothelium is even more permeable Failure to move fluid from the interstitium to peritubular capillary as a result of low plasma oncotic pressure quickly leads to a backup of fluid in the interstitial space Since the interstitial space contains a small fraction of total volume of the kidneys, only a small increase in fluid volume is required to generate a rise in hydrostatic pressure Once interstitial pressure rises significantly, this drives an increasing back-leak The answer is false Not all solutes are alike osmotically Proteins, eg, exert far more osmotic effect mole for mole than do saccharides In addition, saccharides exert a somewhat higher osmotic effect than simple salts In this text, we sometimes simplify things by using osmolarity, when technically we should use osmolality In most cases, this does not introduce a large error Despite the volume flow, most solutes are close to diffusional equilibrium between plasma and interstitium If the interstitium starts to become depleted of a substance as a result of secretion, net diffusion from the plasma will soon replenish it

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