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JPEG takes a different approach. JPEG, in case you are curious, stands for the Joint Photographic Experts Group, the name of the group that created the standard. With JPEG, you get to keep all your colors, but you don t get to keep all the data about the image. What kinds of images lend themselves to being compressed with JPEG A tree. If you take a photo of a pine tree, the acorns are in specific places, but when the image is compressed and decompressed (opened on your Web page), the computer has to approximate where those acorns went, because it had to throw away some of the data. Is this a problem Not with most photos of most pine trees. Faces also take well to JPEG because the colors are all there; faces in GIF can look unnatural because of the color loss. Every generation 3 and higher browser can handle inline JPEGs. JPEGs are also ideal for showing gradient filled graphics (when the color changes gradually from one color to another). The same graphic would suffer enormously under the GIF compression because all those in-between colors wouldn t be there. What suffers under JPEG compression Text, schematic drawings, and any line art. Of course, with JPEG, you can select the level of compression (usually either as a percentage or as Maximum, High, Medium, or Low). You generally want to use the maximum compression level your image can handle without losing image quality. You won t know how much compression your image can handle without loss until you try it at different levels of compression.



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Of course, it is possible to use the same content for all terminals by adhering to the lowest common denominator in this case, short textual descriptions. However, the owners of high-capability terminals would not nd the service satisfactory, as it does not take advantage of the features of their terminals. A service that strives to be successful has to provide each terminal with content that adequately utilizes the capabilities of the terminal. Even this complex picture of terminal diversity is a simpli cation of the reality we are facing. For example, of those terminals that support color images, the maximum resolution of supported images varies, as do supported image formats and the maximum size of multimedia messages that the terminal can receive. Similarly, the processing power of a phone affects how high-resolution videos it is able to play or the complexity of the games it can offer. However, terminal diversity is only one part of the problem. The bandwidth available to terminals varies, both because of terminal capabilities (whether it supports Bluetooth, GPRS, WLAN, or UMTS) and because of network characteristics and coverage. Thus, at different times different content might be suitable even for the same terminal. For example, when a user is outside densely populated areas, there is likely no UMTS coverage and only GPRS is available. In this case, a high-quality streaming audio or video should be replaced by a lower-quality streaming to meet bandwidth limitations. Reducing the quality of Webpages for low-band-





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that a 5ESS time slot has 16 bits (see Figure 6.23).

Spreading basins, which are open pits excavated into the aquifer materials; clearly these are only applicable where the aquifer lies close to the surface. The water will later be pumped from the aquifer using water wells located beyond the margins of the spreading basin. Injection wells, which can be used to introduce water into aquifers at virtually any depth. In many cases, injection wells can also serve as pumping wells in the seasons of high demand.

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2.2. Calcium- and Bicarbonate-Free Hanks Balanced Salt Solution with HEPES (CBFHH) NaCl 136.9 mM, KCl 5.36 mM, MgSO4 (H2 O)7 0.81 mM, glucose 5.55 mM, KH2 PO4 0.44 mM, Na2 HPO4 (H2 O)7 0.34 mM, HEPES 20 mM, adjusted to pH 7.5 with NaOH (all chemicals are of analytical grade or best commercially available). Sterilize by ltration. 2.3. Tyrode s Solution (0.2 mM and 0.4 mM Ca2+ ) NaCl 119.8 mM, KCl 5,4 mM, MgCl2 1.05 mM, CaCl2 0.2 mM or 0.4 mM, NaH2 PO4 0.42 mM, NaHCO3 22.6 mM, glucose 5.05 mM, Na2 EDTA 0.05 mM, ascorbic acid 0.28 mM. Maintain pH at 7.4 by bubbling with 95% O2 5% CO2 (carbogen). 2.4. Trypsin Stock Solution (0.1 g/ml; 50 ) Dissolve 5 g in 50 ml of CBFHH overnight with gentle shaking. Sterilize by ltration. Dilute 1:50 with CBFHH for use. 2.5. DNase Stock Solution (2 mg/ml; 71 ) Dissolve 100 mg in 50 ml of NaCl (0.15 M). Sterilize by ltration and store at 20 C. Dilute 1 ml with 70 ml of CBFHH for use at 28 g/ml. 2.6. Concentrated DMEM (2 ) DMEM made up at double strength from powder and lter sterilized, or from 10 concentrate. 2.7. Histologic Fixative Formaldehyde 4%, methanol 1%, in phosphate-buffered saline (PBS), pH 7.4, containing 1 mM CaCl2 and 30 mM 2,3-butanedione monoxime (BDM). 2.8. Supplemented Tris-Buffered Saline (STBS) Tris HCl 0.05 M, NaCl 0.15 M, pH 7.4, containing 10% fetal calf serum, 1% bovine serum albumin, 0.5% Triton X-100, and 0.05% thimerosal. 2.9. Glutaraldehyde Fixative Glutaraldehyde 2.5% in PBS, pH 7.4 containing 1 mM CaCl2 and 30 mM BDM 3. ISOLATION AND CULTURE METHODOLOGY

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