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If one item has an option value of 1 and another has a value of 2, the second item will get twice as much space as the first The flag argument is a bitwise OR of several values that tell the sizer the border type to use around the item and what it should do with extra space along the opposite, or secondary, orientation The border can be any combination of wxTOP, wxBOTTOM, wxLEFT, or wxRIGHT (wxALL puts them all together for you) For example, if you want a blank border around the top and left sides of your widget, you could use a flag of wxTOP | wxLEFT If the flag value contains wxGROW (or wxEXPAND), the item will grow to fill the available extra space A value of wxSHAPED means that it will grow proportionally so that it always maintains the original aspect (width-to-height) ratio.



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Instead of growing, the item can remain aligned against a side (by using wxALIGN_LEFT, wxALIGN_ CENTER, wxALIGN_RIGHT, wxALIGN_TOP, or wxALIGN_BOTTOM) The border argument is the number of pixels of padding around the item, and it makes sense only if the flag argument specifies one or more borders (such as wxTOP)..





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As discussed above, the greatest source of error in NIR calibration is usually reference laboratory error, sample nonhomogeneity, and nonrepresentative sampling in the learning (training) set or calibration set population. Instrument quality and equation selection account for only a fraction of the variance or error attributable to NIR analytical technique in current routine application. When radiation is re ected from solid matter surfaces, diffuse and specular re ected energies are superimposed. The intensity of diffusely re ected energy depends on the angles of incidence and observation but also on sample packing density, crystalline structure, refractive index, particle size distribution, and absorptive qualities. Thus, in practice, an ideal diffusely re ecting surface can only be approximated, even with the nest possible grinding of the samples. There are always coherently re ecting surface regions acting as elementary mirrors whose re ection obeys Fresnel s formulas. Radiation returning to the sample surface from the interior can be assumed to be largely isotropic, thus meeting the requirements of the Beer Lambert law. It is assumed that radiant energy is continuously removed from the incident NIR beam and converted to thermal vibrational energy of atoms and molecules. Decrease in the intensity of the diffusely re ected light depends on the absorption coef cient of the sample. The absorption coef cient (K), when taken as the ratio K/S, where S is the scattering coef cient, is proportional to the quantity of absorbing material in the sample. In the Kubelka Munk theory, re ectance (R) is related to absorption (K) and the scattering coef cient (S) by the equation

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3. LABELED PHAGES AS THERAPEUTIC AGENTS The phage capsid has been decorated with bactericidal proteins as an alternative to the genetic modi cation of phage chromosomes to carry toxic genes. One novel study evaluated the use of targeted photosensitizers as bactericidal agents. Light-activated antimicrobial agents (photosensitizers) represent promising alternatives to antibiotics for the treatment of topical infections. To improve ef cacy and avoid possible damage to host tissues, targeting of the photosensitizer to the infecting organism is desirable, and this has previously been achieved using antibodies and chemical modi cation of the agent. Embleton et al. (2005) investigated the possibility of using a phage to deliver the photosensitizer tin(IV) chlorin e6 (SnCe6) to S. aureus. SnCe6 was covalently linked to S. aureus phage 75, and the ability of the conjugate to kill various strains of S. aureus when exposed to red light was determined. Substantial reductions in the concentrations of methicillin- and vancomycin-intermediate strains of S. aureus were achieved using low concentrations of the conjugate (containing 1.5 g/ml SnCe6) and low light doses (21 J/cm2). Under these conditions, the viability of human epithelial cells (in the absence of bacteria) was largely unaffected. On

It then loops through each child, using $child->nextSibling to retrieve the next child in the list. (If there are no more children, nextSibling contains null and the loop exits.)

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obese when fed a high-fat diet (Bruning et al., 2000), indicating that insulin receptor signalling in the brain is important in restraining food intake and body weight (Figure 6.12). Insulin apparently acts through the same hypothalamic neuropeptide systems as leptin, inhibiting the NPY neurones and stimulating the POMC neurones in the ARC (see Figure 6.11). ICV insulin also enhances the satiating effect of peri-pheral CCK (Riedy et al., 1995), but unlike leptin, insulin also reduces nocturnal meal frequency, indicating some divergence in the mechanisms underlying the two hormones effects.

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