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Reagents and Materials Sterile Culture medium (See Section 3.1.2) Filter well inserts, 23 mm, 4- m porosity, polyethylene terephthalate (PET) Multiwell plate, 6 well, deep well Scalpel Protocol Seeding (a) Add 15 ml culture medium to each well of the 6-well deep-well companion plate before positioning the cell culture insert. (b) Resuspend the cells in 5 ml culture medium. (c) Pipette the 5 ml cell suspension onto the 0.4- m inserts in the 6-well plates. (Note: inspect inserts for holes before adding the cell suspension.) (d) The cell suspension should be allowed to settle on the insert for 1 full day without movement before changing the medium. (e) Change the medium every other day after the rst 2 days (15 ml outer well, followed by 5 ml to the insert). (f) Collect inserts after 2 3 weeks. Maintaining Cultures of ARC Tissue (g) Remove medium from both the bottom of the deep-well plate and the lter well insert.



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Lerat et al., 2005

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Using the Job Queue parameters, you can influence the runtime operation of scheduled jobs on this frontend client from automatic commercial detection to the detection method used. Commercial Flag New Recordings Every time you schedule a new recording, MythTV is right there with you, flagging for commercials (by default). Disable this feature to conserve CPU overhead. Commercial Skip Method By default, this option functions only with Blank Frame Detection set, but when you enable the preceding checkbox, a variety of other options suddenly become available. The other features enable you to specify the many methods





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and contains information describing RTP s payload type. Information describing the payload type allows RTP to support multiple compression types such as MPEG and H.261. In fact, an RTP receiver can receive information that is encoded by two different methods and has the ability to produce one single stream from this. This is a process known as mixing. It is explained later. A digitized audio signal may be produced using an simplex encoding scheme known as pulse code modulation (PCM). Say, for example, that PCM builds 160 byte packets every 20 milliseconds for a sampled voice stream. This information is transmitted through an Internet using IP. The digitizing of the voice signal is very sensitive to time. The reception of the stream of voice must be put back into the original timing in which it was transmitted; otherwise, there will be an uneven flow for voice at the receiver and it will not be received as it was spoken at the originator. IP does not care about timing, sequencing, or delays. It only has to deliver the data. IP will probably deliver these packets at different times and may deliver them in varying order. Therefore, an RTP application must put the packets back in the original order and reapply the timing between the packets. RTP provides information on this but does not accomplish this directly. Timestamps, which mark the relative beginning of the event, are provided with the packet and this provides enough information to the application to rebuild the original audio stream.

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Multiple responses on the same scale (eg, pretest, posttest, and follow-up scores on a measure of depression) are generally considered to produce univariate statistical designs (eg, within-subjects ANOVA), although they are sometimes treated multivariately Having multiple responses complicates data screening because there are also relationships among those responses to consider Examining the Variables or the Residuals of Analysis Another issue is whether the examination of assumptions is performed on the raw variables prior to analysis or whether the main analysis is performed and its residuals examined Both procedures are likely to uncover the same problems For example, a peculiar score (an outlier) can be identi ed initially as a deviant score in its own distribution or as a score with a large residual that is not t well by the solution Temptation is a major difference between these two alternatives.

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