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Last but not least, the possible application of a certain SBS material may be restricted by toxicity or other safety reasons caused by the properties of the material Therefore, from the large list of possible and known SBS materials, only a small number are prominently used in laser applications One is Freon-113 and other derivatives of this class of organic liquids SF6, nitrogen, and xenon are frequently.

Eggleston, T J Kane, K Kuhn, J Unternahrer, and R L Byer, The slab geometry laser Part 1: Theory, IEEE J Quantum Electron 20, 289 301 (1984) 27 T J Kane, J M Eggleston, and R L Byer, The slab geometry laser Part II: Thermal Effects in a Finite Slab, IEEE J Quantum Electron 21, 1195 1210 (1985) 28 L A Hackel and C B Dane, High power, high beam quality regenerative ampli er, US Patent No 5,239,408, August 24, 1993 29 J Miller, L Hackel, C Dane, and L Zapata, High power regenerative laser ampli er, US Patent No 5,285,310, February 8, 1994 30 L F Weaver, C S Petty, and D Eimerl, Multikilowatt Pockels cell for high average power laser systems, J Appl Phys 68, 2589 2598 (1990) 31 I M Thomas, High laser damage threshold porous silica antire ective coating, Appl Opt.

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A process likely to result in microheterogeneity within the sample will tend to hinder crystal formation; heterogeneity interferes with orderly recruitment into and ef cient packing within the crystal. Frequently, it is easier to crystallize a protein in the presence of a low molecular weight ligand as this reduces conformational variability within the population of sample molecules. This process is referred to as cocrystallization. Good examples are cocrystallization of enzymes with inhibitors or of hormone receptors with hormones. The necessity for ultrapure sample also leads to a requirement for particularly low batch-to-batch variation during protein puri cation or oligonucleotide synthesis for crystallographic work. The general approach taken to crystallization is to expose a concentrated aliquot of a sample of protein/DNA to a concentration of precipitant which is slightly below that necessary to precipitate the sample. The most widely used precipitants are low molecular weight poly-alcohols (e.g. hexane diol), salts (e.g. ammonium sulfate), high molecular weight polymers (e.g. polyethylene glycol) and organic solvents (e.g. methanol). At the beginning of the experiment, the sample solution, though concentrated, is still unsaturated (see the phase diagram in Figure 6.31). During the experiment the protein and/or precipitant concentration is increased slowly such that eventually the saturation point of the phase diagram is reached. This is the point where the solid and liquid states of the protein exist in equilibrium

25, 1481 1483 (1986) 32 C B Dane and L A Hackel, Long-pulse-width narrow-bandwidth solid-state illuminator laser, US Patent No 5,689,363, November 18, 1997 33 G C Valley, A review of stimulated Brillouin scattering excited with a broad-band pump laser, IEEE J Quantum Electron 22, 704 712 (1986) 34 A A Filippo and M R Perrone, Experimental study of stimulated Brillouin scattering by broad-band pumping, IEEE J Quantum Electron 28, 1859 1863 (1992)..

If you don t see a line like this, add it, and then save the file. Restart inet.d by giving the following command:

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Doug LaMaster (age 12) has generously created the SWF file shown in the following steps for you to learn and experiment with. You can find it in the Exercises folder, under this chapter number.

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