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In addition to the Pen tool, Illustrator contains a Paint Brush tool. The brush s biggest advantage is not the ease with which you can draw by simply clicking and dragging with the mouse, but rather the fact that you can use it with a wide variety of brush tips to create anything from simple strokes to painted shapes (see Figure QS.5). Illustrator ships with nearly 30 brush libraries, each containing from a few brushes to dozens of them (see Figure QS.6).

By contrast, coumarin derivatives are useful for shorter UV wavelength excitation, as are pyrenes such as cascade blue (Figure 429) One extrinsic uorophore has proven particularly useful for the probe labelling of oligonucleotides or even nucleotides, namely N-methylanthranilic acid (MANT) This is considered a small probe prepared from the alcohol-reactive probe precursor N-methylisatoic anhydride, which appears to combine with 2 -hydroxyl groups of -d-ribofuranose rings, introducing the uorescent MANT moiety without appearing to impair the functional binding behaviour of oligonucleotides or nucleotides (Figures 429 and 430) Increasingly, the tendency in uorescent probe design has been to make designs exible in order that precise wavelengths of absorption and emission can be tailored to requirements by subtle changes in selected structural parameters For instance, Alexa Fluor probes can be tailored to cover a huge range of wavelengths and can be very good choices in many kinds of application.

=ADDRESS(ROW(Data),COLUMN(Data)+MATCH(Target,Data,0)-1)

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The most widely used Alexa Fluor dye is Alexa Fluor 488 (the number represents the excitation wavelength).

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refresh task is considered an entry to the card. At that time, the timer SHALL restart. Note: Variables may be used to simulate resuming a timer.

1. Open the database in FileMaker Pro. 2. Choose File Save a Copy As. A standard file dialog appears. (See Figure 17-4.) 3. Choose Clone (no records) from the Type pop-up menu/Save a drop-down list. 4. Select a destination disk and folder by using normal file navigation procedures. 5. Type a name for the clone in the Save as (Mac OS X) or File name (Windows) text box. If you re saving the file in a different folder and/or disk than the one where the current database is stored, you can use the same name as that of the original database. If you re storing it in the same folder and/or disk, you ll want to use a new name or the default name proposed by FileMaker Pro (filename Clone.fp7).

If you re dealing with a wide range of fractions in something like a cookbook, you probably won t find all the fractions you need in your Expert Collection font. Regular Type 1 Postscript and TrueType fonts certainly don t have the desired fractions; and although OpenType fonts have the necessary characters to create almost any fraction, many fonts are not available in OpenType format and even when they are available, many publishers have not spent the money to replace them all. Therefore, most organizations opt for formatting all the fractions manually. However, if you are a frequent fraction user, I strongly encourage you to switch to OpenType fonts. The built-in fractions in expert fonts and the fractions generated by OpenType fonts are approximately the same size as a single character in that font; this size should be your goal in formatting a fraction manually. Usually, you achieve this by decreasing the size of the two numerals (perhaps by using the subscript and superscript formats), raising the numerator (the first, or top, number in the fraction) using the baseline shift feature, and kerning on either side of the slash as necessary. ( 20 covers these controls.)

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