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Learn how to convert PDF to Word in 5 simple steps with Adobe Acrobat DC. You can easily convert your PDF files to editable Word documents. Start with free ... This bioreactor system represents a well-characterized platform in which O2 gradients can be regulated by altering inlet concentration and ow rate. As expected at higher ow rates ( 1.0 ml/min), given an inlet concentration of 76 mmHg, O2 tension does not fall to perivenous levels in the length of the ow chamber. Increasing inlet O2 can also prevent the decrease to the perivenous range. Notably, previous strategies for bioarti cial liver devices have utilized preoxygenation of inlet streams to supraphysiologic levels in order to prevent inadequate O2 delivery in parts of the bioreactor [Custer and Mullon, 1998]. However, as we have illustrated, O2 can modulate hepatocyte function and therefore constitutes an important design criterion for an effective bioreactor system. Another important design issue for bioreactors is the shear stress experienced by the cultured cells [Ledezma et al., 1999]. In the system described above, a ow rate of 0.5 ml/min corresponds to 1.25 dyn/cm2 , which is well below the shear stress of 5 dyn/cm2 that was previously demonstrated to decrease rat hepatocyte function [Tilles et al., 2001]. Consequently, in our system, a ow rate of 0.5 ml/min and inlet of 76 mmHg represent the ideal parameters for preventing cellular damage due to shear stress or hypoxia, and concurrently generating adequate O2 concentration gradients for the establishment of hepatocyte zonation. convert pdf to word text online free Convert PDF to Word - Free PDF to Word Converter Online
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