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bacteria and the trend against the use of antimicrobials in the food production chain seem to recommend a reappraisal of the use of phagebased therapies In 2000, the American National Institutes of Health released a challenge-grant announcement that speci cally called for proposals using phage therapies for emerging and resistant infections, placing special focus on vancomycin-resistant enterococci and multidrug-resistant staphylococci A nal question merits consideration.

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3. Use the fact that { fn}p=l H,' (R) a consequence of the Poincark-Friedrichs' C and inequality to simplify the convergence analysis.





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Would it be justi able to patent a phage More importantly, would the pharmaceutical industry be willing to invest the money in research and development into such matters and accept limited patent protection The use of phages has several advantages: (i) phages are self-replicating; (ii) they are more speci c than antibiotics and hence do not cause damage to the normal microbiota; (iii) they have few side effects; (iv) they are alternatives for patients with allergies to antibiotics; (v) they have low production costs; (vi) they might be used for prophylaxis in hospital settings; (vii) their administration can be carried out through different routes; (viii) they may exhibit synergistic effects with antibiotics or even other medicines; (ix) whole-phage particles are able to replicate at the site of infection and so concentration occurs only where they are needed; and (x) the search for and development of new alternatives against resistant bacteria is faster than the development of new antibiotics.

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In this section we discuss higher-order Gaussian numerical quadrature rules on the reference domains K,and Kt . For details regarding the theory and open problems in modern Gaussian numerical quadrature, we refer to [35,46, 47, 48, 49, 70, SO, 104, 1081 and [ 1 141. For practical implementation, CD-ROM containing Gaussian quadrature data for various 2D and 3D reference domains and polynomials of the degree up to p = 20 is part of [ 11 13. With the quadrature rules available on the reference domains K f and K,, quadrature the on arbitrary quadrilateral or triangular mesh elements is performed via the Substitution Theorem,

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Evidently, antibiotics have certain advantages over bacteriophages, the main one being that they show a broad spectrum of action, such they can be used when the exact nature of the disease-causing bacteria is unknown However, this wasteful use has also been responsible for the increase in antibiotic resistance 23 Therapy with Phage Enzymes The development of molecular techniques has allowed a new approach to bacteriophage-based therapy: the use of puri ed lytic enzymes or holins alone without the need of the whole phage particle Indeed, in this sense, the ef cacy of lysins against a variety of causal agents has been demonstrated The cloned enzyme PlyG from phage can kill Bacillus anthracis strains, such that when it is administered in vivo the enzyme readily protects mice from B anthracis infections (Schuch et al 2002) The success of lytic enzymes has been demonstrated against infections caused by vancomycin-resistant E.

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Figure 10.5 Schematic representations of (a) time to contact for an object approaching a stationary observer ( local tau 1) and ( b) final place of contact for a stationary observer.

faecium and vancomycin-resistant E faecalis (Biswas et al 2002; Yoong et al 2004)..

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