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Argue that the contractivity requirement J~FNJI be met if the step-size parameter cy < 1 can satisfies max, 11 - aF(ejw)l < 1,where F(ej") denotes the frequency response of F ( z ) . (e) Assume v ( i ) = 0 and let e = { m e n ( 0 ) ,m e n ( l ) ,. . . , m e n ( N ) }which, as Under the same conditions of part (d) show seen from part (b), is the input to the mapping FN. that l l G ~ 1 1 ~ 5 IliU-111~ ll(I - aCN)1)2))e(12 Ilell'. Argue that an approximate choice for a that maximizes the decrease in energy from llG-111' to l l G ~ 1 can be obtained by solving 1~ min,, maxw - aF(ej")( over values o f a that guarantee max ( 1- aF(ejw)l < 1.



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(Loef er et al 2001), vancomycin-resistant Enterococcus faecalis and Enterococcus faecium (Yoong et al 2004), as well as methicillin-resistant S aureus (MRSA; O Flaherty et al 2005) and S aureus strains with reduced susceptibility to vancomycin (Rashel et al 2007) It was also shown that lysins can act synergistically with other lytic enzymes and antibiotics (Loef er and Fischetti 2003; Djurkovic et al 2005; Becker et al 2008) 211 Lysins Speci c to S pyogenes The rst and hitherto only lysin speci c to S pyogenes that was evaluated as a potential antibacterial agent was PlyC amidase derived from group C streptococci C1 phage (Nelson et al 2001; Nelson et al 2006) This enzyme is very interesting in at least two respects First, it is the most potent lysin reported so far, its activity being over two orders of magnitude higher than those of other bacteriophage lytic enzymes.





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Second, PlyC is the only known multimeric lysin, while all the others are synthesized as single polypeptides Although PlyC was rst reported in 1957, it was not until 2001 that its antibacterial activity was studied in more detail both in vitro and in vivo In fact, it is the rst lysin whose activity was studied with a view to potential prophylactic or therapeutic use In vitro experiments revealed that, unlike C1 phage, the enzyme lyses S pyogenes most ef ciently, while its activity against groups C and E is substantially lower All 10 S pyogenes strains tested were ef ciently lysed by PlyC On the other hand, the lysin practically did not act on streptococci groups B, D, F, G, L, and N or other bacterial species with the exception of Streptococcus gordonii, which was lysed very slowly.

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Furthermore, footnotes are included in order to provide some information regarding the reasons for the choices that have been made in lling in the tables. Table 10.5 is presented to allow the reader to make his/her own choice from the whole range of technical solutions. It is possible to choose personal coef cients for accuracy, infrastructure complexity, as well as terminal complexity. The following terms are used:

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Such an antibacterial range appears to be very advantageous because it is essentially limited to pathogenic streptococci (groups A and C) In a murine model of oral colonization, a single dose of the lysin administered to the oral cavity of mice prior to 107 colony forming units (cfu) of group A streptococci resulted in signi cant protection from the mucosal colonization (only 285% of the mice that received PlyC were colonized compared with 705% of the animals in the control group) Importantly, in most mice that were colonized despite administration of enzyme, cfu counts remained low throughout the experiment or the bacteria were completely eliminated within 48 h, whereas those in the control group increased during the same period of time In another experiment, no streptococci were detected in oral swabs of nine heavily colonized mice 2 h after administration of one lysin dose.

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