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Our laboratory has developed several distinct model systems that recapitulate critical structural and/or functional aspects of the liver Speci cally, as a means to investigate systematically the role of heterotypic contact in hepatocyte cocultures, our laboratory has developed and characterized a versatile micropatterning method, which has enabled the quantitative control of the spatial relationship of hepatocytes and broblasts [Bhatia, 1997] This method is one in a repertoire of techniques that we have developed to control cell-cell interactions These processes enable an examination of the mechanisms of interaction, including the potential importance of numerous aspects such as continuous signaling, gap junctions, and soluble factors Furthermore, the micropatterning method described in this chapter also represents a robust platform for the in vitro functional stabilization of hepatocytes.



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The shortcuts are slightly different when you re working on a grayscale image. You access the image itself by pressing Ctrl+1 ( -1 on the Mac). Ctrl+2 ( -2 on the Mac) and higher take you to extra spot-color and mask channels.





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Moreover, to gain another level of control of environmental in uences and further enhance the physiologically relevant function of hepatocytes in vitro, we have also developed additional approaches First, we have recently described the construction of a perfusion bioreactor system that has enabled the formation of steady-state oxygen gradients, resulting in the presence of zonated hepatocyte characteristics [Allen and Bhatia, 2003), which is an important feature of the liver in vivo For example, numerous drugs exhibit zonal toxicity due to compartmentalization of drug-metabolizing enzymes [Lindros, 1997] As a result, the in vitro model of zonation described here represents a unique system to investigate, among many aspects, the mechanisms of zonal hepatotoxicity Each of these approaches (micropatterning and bioreactor systems) recapitulates two-dimensionally certain aspects of hepatocyte function.

is also 1 on average,

(38.24) P~ } The coefficient matrices { PM,~, , i - 1 that appear in the above expression have the same order M . Dropping M , we can rewrite the above relation as

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In many instances, 2-D systems are ideal because they minimize complexities in aspects such as imaging and molecular, histologic, or immunohistochemical characterization, often introduced in 3-D culture models In contrast, for implantable systems, the development of 3-D constructs is required Numerous reports have examined the seeding or recruitment of cells to prefabricated 3-D scaffolds [Badylak et al, 2001; Langer and Vacanti, 1993; Lee et al, 2000; Yang et al, 2001] However, particularly for hepatocytes, which do not grow or migrate well in vitro, the development of 3-D systems would require the formation of thick constructs containing a homogeneous.

t some time or another, the files you output to your desktop printer may be candidates for offset printing. The tools and commands you use for desktop printers, as I explained in 31, are sufficient for printing to all the printers you have in your office and home. However, when printing PDF documents on commercial printing devices, you must address a number of issues. For high-end digital prepress and commercial printing, Acrobat Professional includes all the print controls desired by the commercial printing community. Combined with features for previewing color, preflighting jobs, and printing color separations, Acrobat Professional ranks as a strong competitor against any layout or other professional applications designed to serve creative professionals. In this chapter I cover printing from Acrobat Professional using many commercial printing tools.

The final topic of this chapter on capital assets concerns asset impairments. The GASB recently addressed this issue with the issuance of Statement No. 42, Accounting and Financial Reporting for Impairment of Capital Assets and for Insurance Recoveries. This new GASB statement requires governments to

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The best way to understand painting in Photoshop is to look at the brush-based tools that Photoshop provides. Understanding how these painting tools work can make you more effective at both editing photos and applying artistic effects. This section discusses the painting tools and how to use them. You use three types of tools to paint in Photoshop: editing, painting, and mixing. Editing tools paint by adjusting the pixels directly beneath the tool. Painting tools paint by applying color to the pixels directly beneath the tool. Mixing tools paint by mixing color with the pixels directly beneath the tool.

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