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Our online PDF editor makes editing PDFs as easy as editing Word documents. Now you can edit pdf text online free & easily . Merge, split, delete, modify PDF ... oligonucleotides and lipids. Other highly selective bioconjugations to probes are made possible in oligo- or polypeptides in other ways. For instance, N-terminal serine or threonine residues of an oligo- or polypeptide can be selectively oxidised by oxidative cleavage to give aldehydes (Figure 4.20), which are primed for bioconjugation to uorescent probes with amines or hydrazines. Furthermore, tyrosine residues can sometimes be selectively modi ed by ortho-nitration using tetranitromethane followed by reduction to form an aminotyrosine (Figure 4.21). Alternatively, a particularly innovative approach is to use a puri ed enzyme (transglutaminase) to speci cally modify glutamine residues with amine-containing probes in the primary amide functional group (Figure 4.22). The presence of an aliphatic spacer between the amine functional group and the uorescent probe increases reaction ef cacy, especially when a cadaverine spacer is used (as shown). One extreme view of chemical introduction of an extrinsic uorescent probe is found in the case of the alanine derivative of the uorophore 6-dimethylamino-2-acylnaphthalene (DAN) (Figure 4.23). This derivative uorophore, given the trivial name Aladan, is incorporated into a polypeptide by solid-phase synthetic chemistry (although a molecular biology technique known as nonsense suppression is now available for the introduction of unnatural aminoacid residues into recombinant proteins). The uorescent emission maximum (Imax ) of Aladan shifts dramatically on different solvent exposures, from 409 nm in heptane to 542 nm in water, yet at the same time remains only mildly changed by variations in pH or salt concentration. This compares to a maximum environment-mediated shift of around 40 nm for intrinsic tryptophan uorescence. In addition, there is little spectral overlap between extrinsic Aladan uorescence and intrinsic uorescence from tryptophan or tyrosine. pdf editor software online purchase Free PDF Editor & Free PDF Form Filler - PDFescape
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