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What is the current knowledge about nonspeci c adsorption of biomolecules in the particular case of diamond surfaces Several groups have so far investigated the adsorption of proteins on diamond surfaces Hydrophobic H-terminated nanocrystalline diamond surfaces have been reported to exhibit a low adhesion of plasma proteins, such as brinogen [60, 61] On the other hand, hydrophilic O-terminated nanocrystalline diamond surfaces were shown to provide an exceptionally high af nity for proteins [62] Steinm ller-Nethl u and coworkers reported that O-terminated NCD is extremely ef cient in adsorbing the bone morphogenetic protein-2 (BMP-2), due to the combination of suitable surface texture and hydrophilicity [63] Interestingly, bound BMP-2 was found to be fully active, which shows that simple physisorption can be useful for medical implants as well as for innovative scaffolds in tissue engineering [63].



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6 Jun 2015 ... Here Mudassar Ahmed Khan has explained how to open (view) PDF Files on Browser in ASP . Net using C# and VB.Net. This article will explain ...

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Response.ContentType = "Application/ pdf "; Response.TransmitFile(PDFfilepath); . For opening the PDF file in a new tab or windows you can ...

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Similar results were obtained using oxidized diamond nanocrystals, with an excellent af nity for different proteins; lysozyme, for instance, strongly adsorbs on O-terminated nanodiamonds while maintaining its enzymatic activity [62] Thus, it seems the case that for the particular case of diamond surfaces the lower the wettability the weaker the adsorption However, this is in contradiction with the previously discussed report of Whitesides and coworkers [70], which demonstrated that hydrophobic surfaces are more prone to nonspeci c adsorption of proteins Therefore, these results suggest that in the case of diamond surfaces wettability is not the only parameter to consider Other factors such as electrostatic interactions are able to in uence the adsorption substantially In contrast to other materials, the charge of diamond surfaces in contact with aqueous electrolytes has not been investigated in detail.

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Recently, we have studied the charge build-up at H-terminated and O-terminated diamond surfaces in aqueous electrolyte using electrokinetics experiments, from which the zeta potential could be calculated [71] The zeta potential is commonly used for quanti cation of the electric charge at the double layer present at, for instance, the solid/liquid interface It can be de ned as the potential at the boundary between the immobile layer of adsorbed ions on a surface and the diffuse layer of mobile ions, ie the potential at the so-called shear or slipping plane [72] However, it is important to realize that the zeta potential differs from the electric surface potential in the double layer Yet, it can provide a good estimation of the electric charge of a surface in contact with an electrolyte Figure 1613 shows the pH dependence of the zeta potential of H-terminated and O-terminated diamond surfaces.

If the <img> tag does not contain an alt attribute, some screen readers read the filename when they encounter the image, which slows down how quickly visually impaired users can get to the real information on your page. For images that are purely visual and don t contribute to the meaning of your content, such as bullets or spacer images, include a blank alt attribute. To do this, open the Image property inspector and select <empty> from the alt drop-down list.

The leading men were accompanied by ladies acting as secretaries, wives and who knows what else. They were from Japan, Malaysia, Hong Kong, Indonesia, Taiwan and the Philippines. They were hard workers and would send e-mails, call clients using Sherwood s Voice over Internet Protocol (VoIP) phone system and take messages for their bosses.

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The isoelectric point for O-terminated surfaces was found to be close to pH 15 On the other hand, the isoelectric point for the H-terminated surfaces was found to be slightly below pH 4 This means that at the physiological relevant pH, both surfaces are negatively charged The negative charge at the O-terminated diamond surface could be induced by carboxyl or hydroxyl surface groups, as has been observed for metal oxides More surprising is the negative charge observed at the H-terminated surfaces, where the number of those groups should be very small Similar results have been observed for other hydrophobic surfaces, in which the measured isoelectric point was about pH 4 [73] Thus, hydrophobic surfaces in contact with water acquire a net negative charge at physiological pH.

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