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weaker than the energy of transfer of the azobenzene into the nanoparticles In other words, a regime of abrupt saturation of the particles is reached when the distance between azobenzenes in the chain is decreased down to the critical value of loop length The critical transition occurs at Floop=Fbinding The degree of integration of the azobenzene in the polymer de nes the length of the shorter possible loops, and in turn controls the regime of association In the regime of tight multipoint association, the saturation should, however, be reached when either all the binding sites on the particle (eg, number of hydrophobic pockets per protein) are associated or when interloops repulsions damper the energy of association.



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wprintw(test, _maxx = %d, _maxy = %d\n ,test->_maxx,test->_maxy);

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To estimate the typical number of loops that can be associated on a micelle, we consider that the maximal coverage is almost reached when the loop density brings the bound polymer segments into contact [NloopB4 (1+Rmic/R)2, with R being the radius of a loop and Rmic being the radius of micellar core of the order of 1 nm] At integration levels of the azobenzene signi cantly lower than B5 mol% (constrained by the requirement of small absorbance of solutions), the loops typically contain more than 20 monomers and R would be much higher than 1 nm Accordingly, 4 60 loops could be gathered on a single micelle; a value that matches well with experimental data in Fig 77: 1 g/g bound corresponds here to B40 azobenzene per 100 surfactants, with ca 50% unbound azobenzene.

Here, the statement displays at row 11 and the column indicated by COL2, the text October 19, 1993 right-justified in a column 30 characters wide.

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Close to critical transition between the two regimes of association, a small variation of the af nity for the particle triggers the collective detachment of the loops In other words, when free energy of loop formation is close to balance the energy of hydrophobic binding, the trans to cis isomerization is expected to abruptly make the bound particle almost free In our experiments, azo-modi ed polyacrylates containing more than 1 mol% azo were tightly bound to micelles or bovine serum albumin (BSA) in the dark, but the association vanished for chains with lower integration level The binding transition occurs accordingly at a loop length of the order of 100 200, that is, at Floop #5 6 kT This energy is comparable to the energy of hydrophobic association, which can be estimated by B1 kT per hydrocarbon group transferred in a micelle core.

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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 #include <ncurses.h> int main(void) { int yoda = 874,ss = 65; initscr(); printw( Yoda is %d years old\n ,yoda); printw( He has collected %d years\n ,yoda-ss); printw( of Social Security. ); refresh(); getch(); endwin(); return 0; }

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