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Genetic material contains genes many of which encode proteins. This complement of genes is referred to as the genome and can be regarded as a relatively static set of instructions (Figure 10.1). When transcribed, these genes direct synthesis of a dynamic population of mRNA molecules that will vary with the requirements of the cell at any given time. This is referred to as the transcriptome. When translated, the population of mRNAs directs synthesis of a population of proteins that can be further varied by post-translational processing such as glycosylation or proteolysis. This is the proteome, the total complement of proteins expressed in a cell at any given point in time and under a given set of growth conditions. Like the transcriptome, the proteome is a dynamic quantity that can change in response to nutritional status, stress and specialization of the cell or with stage of the cell cycle. Proteomics is the study of the proteome using methodologies capable of analyzing large numbers of proteins simultaneously. Three main types of techniques have contributed to development of the eld of proteomics; electrophoresis, mass spectrometry and chip technologies. These make possible high-throughput analysis capable of revealing alterations in presence or level of individual proteins. These approaches have contributed to development of subspecialties such as descriptive proteomics (cataloguing of proteins), functional proteomics (focusing on the dynamic state of the proteome) and interaction proteomics (exploring protein-protein interactions). This chapter describes these technologies and some of their current applications. The reader should note that some common chemical tools/approaches are used across these methodologies (e.g. use of reducing and alkylating agents; isotope/ uorlabelling; reactions of functional groups with groups on stationary phase beads). Traditional proteomics aimed to



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Properties palette, see the section, Editing with the Quick Properties Palette and the Properties Palette, later in this chapter. When you double-click certain types of objects in a drawing, you see a dialog box that is specific to these objects, or a related editing command starts: n Attribute definition. Opens the Edit Attribute Definition dialog box (the DDEDIT command). See 18 for more information. n Attribute within a block. Opens the Enhanced Attribute Editor dialog box (the EATTEDIT command). See 18 for more information. n Block. Opens the Edit Block Definition dialog box. You can choose the block from a list and click OK to enter the Block Editor. See 18 for more information. n Hatch. Opens the Hatch Edit dialog box (the HATCHEDIT command). See 16 for more information. n Image. Opens the IMAGEADJUST command. See 27 for more information. n Livesection. Starts the LIVESECTION command. See 24 for more information. n Mline. Opens the Multilines Edit Tools dialog box (the MLEDIT command). Mlines are not available in AutoCAD LT, which has a similar feature, called Dlines. See 16 for more information. n Mtext or leader text. Opens the In-Place Text Editor (the MTEDIT command). See 13 for more information. n Spline. Starts the SPLINEDIT command. n Polyline. Starts the PEDIT command. n Text (TEXT or DTEXT commands). Opens the In-Place Text Editor (the DDEDIT command). See 13 for more information. n Xref. Opens the Reference Edit dialog box (the REFEDIT command). See 19 for more information.

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>>> class Five: ... def __int__(self): ... return 5 >>> f = Five() >>> int(f) 5

Function exp10(real) abs(real) rnd(real) trunc(real) r2d(angle) d2r(angle)

The SECTION command creates a 2D region from a cross-section of a 3D model along a plane that you specify. The original objects are left untouched. Figure 24-20 shows a region created using the SECTION command.

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