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Proteins retain their intact native structure when electrophoresis is performed under nondenaturing conditions. This means that enzymes are usually fully active even after electrophoretic separation. Frequently, enzymes are present in multiple forms or isoenzymes in cells. These proteins may be variants arising from a range of post-translational modi cations (e.g. glycosylation) or may be distinct products of speci c genes. After electrophoretic separation, instead of staining the gel with nonspeci c dyes such as coomassie blue, we can often activity stain with enzyme substrates which are speci c for the group of enzymes under investigation. This is called zymogram analysis and it facilitates analysis of individual isoenzymes in cell extracts without the need for their prior puri cation. Isoenzyme expression patterns from such studies can give us useful comparative information on the genetic composition of natural populations of animals, differences between species, tissue-speci c enzyme expression within a single individual and localized expression within a single tissue. A good example of an enzyme present in multiple forms in cells is lactate dehydrogenase (LDH) which catalyses the reduction of pyruvate under anaerobic conditions. This tetrameric enzyme is composed of two types of subunit polypeptides designated H and M. LDH from heart and muscle are composed of four H and M subunits, respectively, but ve isoenzymes are possible in vertebrate liver (H4 , HM3 , H2 M2 , HM3 , M4 ). These isoenzymes are designated LDH-1 to LDH-5, respectively, and are separable by electrophoresis in polyacrylamide gels. Speci c activity staining of these enzymes is possible by the following method;



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The next item available from choices in the Preferences submenu is for Web Capture preferences and the preference choice is available to both Windows and Macintosh users. For more detail on Web Capture and the preferences, see 4 where they are discussed at length. The preference settings in the Web Capture preferences are identical to the options settings that are covered in 4.

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Figure 4.8. Uses of PET in the United States and Canada, 2000 [3].

194. What are the advantages to the bus topology (Section 12.5) 195. What are the disadvantages to the bus topology (Section 12.5) 196. What are the advantages to the star topology (Section 12.5) 197. What are the disadvantages of a star topology (Section 12.5) 198. What are the advantages of a ring topology (Section 12.5) 199. What are the disadvantages of a ring topology (Section 12.5) 200. The (Section 12.5) topology is used for Token Ring and FDDI LANs.

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