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Chromaf n cells have been used for many decades as an easily available model system for studying mechanisms of neurotransmitter synthesis and release, speci cally of cholinergic neuronal or neuroendocrine stimulus-secretion-synthesis coupling Indeed, a recent PubMed search for chromaf n cells (August 2005) revealed some 3900 relevant hits In contrast to bona de chromaf n cells, PC12 pheochromocytoma cells are predominantly dopaminergic, that is, they express tyrosine hydroxylase (TH) and dopamine- -hydroxylase (DBH), and synthesize and secrete small amounts of NE [Greene and Tischler, 1976] In the hands of most (but not all) investigators, PC12 cells do not spontaneously express PNMT or epinephrine, thus suggesting that these cells originated from the NE phenotype of chromaf n cells In the past 25 years PC12 cells have become an enormously popular model, mainly for studying fundamental mechanisms of neuronal differentiation and mechanisms of neurotransmitter synthesis and release.



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Currently (August 2005) there are more than 8500 PubMed citations describing a plethora of physiologic and molecular aspects of these cells, including the inducibility of the expression of functional PNMT [Byrd et al, 1986; Kim et al, 1993; Ebert et al, 1997; Lelkes et al, 1998, Unsworth and Lelkes, 1998b] PC12 cells are in a unique position in that they maintain some of the features of the bipotentiality of fetal/embryonic SA cells, with the added advantage that PC12 cells are readily available and rather easy to culture Thus, depending on the appropriate environmental cues, PC12 cells can differentiate along either the sympathetic neuronal or the neuroendocrine, chromaf nergic pathway For.





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are set to binary 0s. However, the values used to stuff these columns of each STS-1 SPE will produce even parity in the calculation of the STS-1 path BIP-8 (BIP stands for bit interleaved parity). The STS-1 SPE may begin anywhere in the STS-1 envelope capacity. Typically the SPE begins in one STS-1 frame and ends in the next. This is illustrated in Figure 17.4. However, on occasion, the SPE may be wholly contained in one frame. The STS payload pointer resides in the transport overhead. It designates the location of the next octet where the SPE begins. Payload pointers are described in the following paragraphs. The STS POH is associated with each payload and is used to communicate various pieces of information from the point where the payload is mapped into the STS-1

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of the heavy shielding needed for gamma emitters. Finally, uranium-238 is included for purposes of comparison only. While the energy density numbers reported for radioactive materials are extremely attractive, it must be remembered that in most cases the energy is being emitted over a very long period of time. Second, ef cient methods of converting this power to electricity at small scales do not exist. Therefore, ef ciencies would likely be extremely low. Li and Lal [37] have used the 63Ni isotope to actuate a conductive cantilever. As the beta particles (electrons) emitted from the 63Ni isotope collect on the conductive cantilever, there is an electrostatic attraction. At some point, the cantilever contacts the radioisotope and discharges, causing the cantilever to oscillate. Up to this point, the research has only demonstrated the actuation of a cantilever, and not electric power generation. However, electric power could be generated from an oscillating cantilever. The reported power output, de ned as the change over time in the combined mechanical and electrostatic energy stored in the cantilever, is 0.4 pW from a 4 mm 4 mm thin lm of 63Ni. This power level is equivalent to 0.52 mW/cm3. However, it should be noted that using 1 cm3 of 63Ni is impractical. The reported ef ciency of the device is 4 1026.

synchronized, node N1 can directly combine the sensor data from nodes N2 and N3, since the time stamps refer to the same timescale. If the clocks are not synchronized, a timescale transformation on the received time stamps is necessary. The nal result is identical to that of using synchronized clocks. 7.3.6 Time Instants versus Time Intervals

is kept (application, computing and network infrastructures) and all resources (e.g. networks, IT systems, servers, routers, etc.) are managed that are used to deliver and support services required by or proposed to customers. Administrative, network and information technology events and performance information are collected for distribution to other processes within the enterprise. The OMF supports the lifecycle management of services during operation. To perform this, it needs access to information about services stored in the SUF. This fact has to be re ected when modelling the physical distribution of the components, in the system model.

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