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143 APPROACHES 1431 Adherence to Principles of GMP in a Preclinical Developmental Process The standardized MSC propagation should be conducted as a well-planned, consistently documented, and optimized procedure that also minimizes risks of microbiological, particulate and pyrogen contamination by reducing manipulation steps and manipulation time According to current European legislation,2 the principles of GMP should be applied to CBMP when they are manufactured for use in human subjects in phase 1 studies These requirements do not apply to cellular or tissuebased medicinal products used in phase 1 studies according to US legislation3 or to products in the preclinical developmental phase If it is expected that preclinical ndings are to be translated into clinical use rather rapidly, it may be recommended to establish GMP-compliant technology during the preclinical developmental phase of any cell product.



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As a result, this ensures that products are consistently produced and controlled to meet the quality standards appropriate for their intended use or product speci cation The GMP requirements are well described in PIC/S Guide to Good Manufacturing Practice for Medicinal Products and include the implementation of an ef ciently running quality management system, dedicated areas for manufacture of sterile medicinal products complying with GMP, appropriately quali ed and trained personnel, suitable equipment, correct materials, containers and labels, approved procedures and instructions, suitable storage and transport facilities, and a record-keeping system that allows the complete history of a medicinal product to be traced (See http://wwwpicschemeorg) It is a challenge to conduct preclinical research and development complying to GMP as procedures routinely turn out to be much more time and cost intensive than common laboratory-scale research.





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These circumstances can advance either the developmental progress at the expense of quality standards or vice versa It should therefore be decided on a case-by-case basis how closely to adhere to GMP standards depending on the more or less stringent time schedule for the considered clinical use of a CBMP 1432 Ef cient Standardized MSC Propagation Using Low Cell Seeding Density The future use of MSCs in clinical studies may require very high absolute MSC numbers to gain appropriate cell doses (>5 106 /kg body weight) per patient compared to in vivo experimental models with small animals [20] It is consequently advantageous to develop large-scale MSC expansion protocols that allow for the.

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