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So far we have not taken into account the speci c threshold of the SBS material and the acoustic decay time of the sound wave grating. Low thresholds are especially interesting for CW applications. To the best of our knowledge, there has not been



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University of California at San Francisco (UCSF) PACS network, 253 University of California, Berkeley, author at, xliv, liii University of California, Los Angeles (UCLA) author at, xliv, xlviii, liii CAD studies at, 801 clinical experience with PACS at, 599, 608 612 customized software and data at, 645 PACS implementation case study in, 585 586 University of California, San Francisco (UCSF), author at, xliv, xlviii, liii University of Southern California (USC).





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See also Image Processing and Informatics Laboratory (IPILab); USC HCC2 Building Data Center advanced PACS and informatics training schedule at, 904 907 author at, xliv, liii CAD studies at, 801 clinical environment work ow studies at, 863, 864 in clinical evaluation of CAD bone age assessment system, 845 clinical experience with PACS at, 599, 612 615, 616 618 medical imaging informatics training at, 914 921 PACS/imaging informatics training at, 900 909 PACS implementation case study in, 586 587 University research laboratories, 22 UNIX, 271, 296 See also Solaris UNIX OS server in continuous availability PACS design and implementation, 489 UNIX machines, HIPAA and, 536 Unsharpness, 49, 50 Unusable (U) group, in lossless digital signature embedding, 543 Upper extremities, automatic orientation of, 319 US Army MDIS project, 9 See also United States US Army teleradiology project, 5 USC HCC2 Building Data Center, 254 US.

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Department of Veterans Affairs Healthcare Enterprise (VAHE) information system, 687 693 User acceptance in PACS ef ciency analysis, 593 multimedia ePR systems and, 771 User application layer, in grid computing, 654 User friendliness in PACS, 474 of teleradiology operations, 450 451 User interface, with location tracking and veri cation system, 875, 876 877, 878 879, 880 881, 882 883 User interface icons, 374 Users, multimedia ePR systems and, 771 User training, for multimedia ePR MISS system, 766, 768 769, 770 771 US module, interfacing with PACS gateway, 313 314 US OBGYN images, with LDSERS, 546 548, 549.

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Viability Viability of MSCs can easily be determined immediately after trypsinization via trypan blue or 7-amino-actinomycin D (7-AAD) exclusion According to the speci cations developed from our cell culture studies, viability should be >90% In selected exceptional cases a lower limit of 70% viability of total harvested cells may be acceptable Microbiological Testing Sterility testing that detects fungal, anaerobic, and aerobic bacterial and mycoplasma contamination should be performed after each critical manipulation step during MSC culture that is prone to microbiological contamination [34] The crucial bacterial sterility check at the end of last harvesting step cannot be evaluated prior to in vivo application if MSCs need to be applied immediately after propagation due to the duration of the cultures Mycoplasma polymerase chain reaction (PCR) results can be obtained at the day of harvest within less than 6 h.

MycoAlert results are available within less than 1 h at the day of harvest De nitive culture results to exclude mycoplasma contamination are available within two to three weeks and therefore are not applicable for CBMPs with a short shelf life that are planned to be administered immediately after production Endotoxin and Pyrogenicity Testing Endotoxin measurement using the Limulus amebocyte lysate (LAL) assay is typically done as an alternative to pyrogenicity testing for early phase trials For any parenteral drugs, except those administered intrathecally, the FDA recommends that the upper limit for endotoxin be 5 EU/kg body weight/dose The LAL assay method can be applied to the safety evaluation of biological preparations according to existing regulations.

4 We use the LAL assay to substitute for the lengthy delay in microbiological data availability to obtain results prior to the clinical application of the nal product within less than 2 h after harvest Phenotypic Identity of MSC In addition to morphological identi cation by microscopy, the immunophenotypic characterization of MSCs can be done using a broad panel of uorescence-conjugated antibodies directed against surface molecules To date there is no speci c marker uniquely de ning MSCs Therefore a pro le is used to show the expression of certain markers and to exclude the contamination by cells expressing other marker pro les Flow cytometry is recommended by the ISCT to reveal that MSCs stain positive for CD73, CD90, and CD105 and negative for HLADR, CD14, CD31, CD34, and CD45 (Figure 5) [22, 23].

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