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The pump should be capable of a ow rate between 0.50 and 5.00 mL/min. The pump should be a quaternary gradient pump and have a compositional accuracy of 1.5% of the theoretical values for the four channels. The pump should have relative standard deviation (RSD) of 2.0% for six successive readings from a calibrated owmeter. The autosampler should have an injector capable of injecting sample volumes from 1 to 100 L. The injector should have a precision of 1.5% RSD. The injector carryover should be 0.5% of peak area. The autosampler should pick the correct vial. The autosampler should use relays and/or contacts to communicate with the laboratory CDS. Temperature-controlled sample racks should be capable of maintaining samples in the temperature range of 4 15 C ( 3 C). The detectors should operate with a wavelength range from 200 to 800 nm. The detector should have a wavelength accuracy of 2 nm. The detector response should be linear with a correlation coef cient r2 not less than 0.999 over the full dynamic range. The linear range should be up to 2.0 Absorbance Unit Full Scale (AUFS). The column oven should be capable of maintaining a temperature range of 5 C above ambient to 60 C. The oven should be within 3 C of the set temperature and the temperature precision should be 2.0%.



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Figure 6.18 Conversion gain as a function of the phase of the reactive termination at input port at second-harmonic frequency using the simpli ed model of the active device





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In 2005, 800 million people were overweight (BMI 250 299 kg/m2) and 400 million were obese (BMI 30 kg/m2) Obesity is most prevalent in westernized countries (with up to one-third of adults affected), but is increasing in the developing world, especially in urban populations The highest prevalence worldwide is 80%, among certain Pacific Islanders Most countries show progressive increases in the prevalences of overweight and obesity, including the USA, Europe (notably the UK), the Middle East and Asia Obesity is less common in sub-Saharan Africa (except for South Africa) and is increasingly prevalent among urban populations in India and especially China Childhood obesity is already common in westernized countries and is rapidly increasing in the USA, Latin America, Europe, Middle East and affluent Asian populations In 2004, it was estimated that 10% of children worldwide were overweight and that 2 3% were obese.

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A monochromatic detector observes the sum of all the fringe intensities Because the spacing of the fringes for each wavelength of the source is different, the maxima of fringes will align around only one point where the OPD is zero for all wavelengths as shown in Figure 1529a Away from this zero OPD position the observed sum of the intensities quickly falls off as shown in Figure 1529b It is for this reason that fringes are said to be localized The fringe with maximum contrast, the fringe that marks the zero OPD, is called the zero order fringe, and each next fringe of smaller amplitude on either side is called 1 and 1, 2 and 2 order fringe and so on The maximum of the zero order fringe does not need to fall at the maximum of the fringe envelope (see Section 1565).

Factors contributing to the global spread of obesity include the westernized lifestyle, with increased energy intake and declining levels of physical activity Overall, socio-economic status and educational status are inversely related to the prevalence of obesity, but the relationship is complex It is predicted that, by 2015, 16 billion people will be overweight including 700 million obese The greatest increases are forecast in the USA (with 75% of adults overweight or obese), South America, Europe, Central Asia and the Pacific Rim The combined prevalence of overweight and obesity among children could exceed 40% in the Americas, Middle East and North Africa Obesity predisposes to type 2 diabetes, cardiovascular disease, some cancers and numerous other disorders, including osteoarthritis.

It is thought to account for almost 60% of the risk for developing type 2 diabetes, over 20% of that for hypertension and coronary-heart disease, and between 10% and 30% for various cancers Markers of central obesity are more robust indices of obesity-associated health risk than BMI In Caucasians, a waist circumference of 94 cm in men or 80 cm in women indicates increased risk, while values of 102 cm (men) and 88 cm (women) indicate substantially increased risk Relationships between obesity and health risks vary between populations, with Asians being more susceptible Accordingly, BMI risk thresholds are lower than in other populations, with an action point for overweight defined at 23 kg/m2 In 2000, obesity was estimated to cause 25 million deaths and over 30 million disability-adjusted life years (DALYs), mainly due to ischaemic heart disease and diabetes.

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