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The coordinates x , x define a phase space for the motion that we are discussing, and we have shown that the area in this phase space enclosed by an unaccelerated particle is constant. Only a slight modification is necessary to find the invariant appropriate for accelerated motion and this is the subject of the next subsection. The admittance is the phase space area associated with the largest ellipse that the accelerator will accept. From the preceding discussion, we would estimate the admittance as follows. At any point in the accelerator, the maximum value of x is A&. If the half aperture available to the beam is a(s), then somewhere there will be a minimum in a h ) / Then the



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three months may be necessary to narrow the period in which the API can be demonstrated to be stable [7] Marketed API Intended for Storage in Freezer For an API with intended storage in a freezer ( 20 C 5 C), the retest period should be based on the real-time data available at the long-term storage condition of 20 C 5 C [7] Since no accelerated storage condition for a freezer-stored API is proposed [7], storage and testing of a single batch at elevated temperatures of 5 C 3 C or 25 C 2 C for an appropriate time period should be considered to understand the effect of short-term excursions outside the label storage condition during shipping or handling Other storage conditions may be considered with appropriate justi cation for their selection 714.





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A fourth form of immunoelectrophoresis is two-dimensional or crossed immunoelectrophoresis (CIE). This technique involves initial (i.e. rst dimension) electrophoretic separation of antigens in an agarose gel. This is followed by cutting out a section of gel containing the separated proteins (Figure 5.32). A second agarose gel containing antibodies (i.e. second dimension) is formed against this section and electrophoresis is performed at 90 relative to the axis of the rst gel. Precipitin arcs form similar to those of rocket immunoelectrophoresis and these allow quanti cation of several antigens in a single sample.

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3 Stress Studies and Stability-Indicating Methods for Analysis of API Stability Appropriate physical, chemical, biological, and microbiological attributes of the API that are likely to be susceptible to change during storage and are likely to in uence the quality, safety, and ef cacy should be tested The analytical testing performed to evaluate the stability of the API should be stability indicating as de ned above under GMPs for APIs [3; Section 115] The stability-indicating assay accurately measures the active ingredient without interference from degradation products, process impurities, excipients, or other potential impurities In order to develop a stability-indicating method, it is necessary to conduct stress studies on an API Stress testing is carried out on a single batch of the drug substance and the stresses can include acid (eg, 02 N HCl) and base (eg, 0.

2 N NaOH) hydrolysis, temperature (10 C increments above the accelerated stability storage temperature of 40 C, eg, 50 C, 60 C), humidity (75% RH or greater), photolysis (see Section 716), and oxidation (eg, 10% H2O) of the API [7] Hydrolysis is a chemical transformation process whereby an organic molecule RX reacts with water (H2O), resulting in direct replacement of X by OH Among various stresses stated above, hydrolysis could be the most common stress leading to degradation of an API since amides, esters, and salts of weak acids and strong bases are well known to hydrolyze [13] Photolysis is the process whereby chemicals are altered directly as a result of irradiation or indirectly through interaction with products of irradiation [13].

Carbonyl, nitroaromatic, N-oxide function, C C double bond, weak C H bond, supl des, alkenes, polyenes, and phenols are functional groups that may react with light [14] Oxidation reactions depend on several factors, including temperature, light, pH, oxygen concentration, impurities including metal ions (eg, cupric, ferric), and the oxidizable component of the molecule [15] The stress studies should demonstrate that impurities and degradants from the active ingredient do not interfere with the quantitation of the API [12] Stress testing of the API, in addition to validating the stability-indicating power of the analytical method, can also help establish the degradation pathways and the intrinsic stability of the molecule [7] 7144 Evaluation of API Stability Results.

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