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However, scalability is a significant challenge since it is not possible to actively test each service through every possible service access path, i.e. every cell. Consequently, true endto-end deployments of active test solutions are limited to main areas of interests (key cells) and sometimes deployed in the network, for example at the A-interface for voice call management or in the IP core for data services, to provide a scalable solution. An example of Active Service Management approach is illustrated in Figure 7.6.

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rom the very get-go, the InDesign team engineered the software to be extensible meaning you can extend its capabilities by adding more software. To do this, you use plug-ins, which are small software modules often developed by third parties. If you re a Photoshop user, you might be familiar with plug-ins such as Alien Skin s Eye Candy; or if you re a QuarkXPress user, you re probably familiar with the concept of add-on software through XTensions. Even if you re new to publishing, you may have purchased add-on software for your operating system, such as a custom screen saver or virus-protection software. Extensible programs solve the one-size-fits-all nature of most software, letting you customize your tools to your workflow. If InDesign doesn t meet a need of yours such as indexing, table editing, or imposition you can look for a plug-in that does.

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If you perform two Find All operations in a row, the Search panel automatically clears the results of the first search and replaces them with the results of the new search. To manually clear the Search panel, right-click (Control+click) and choose Clear Results.

The selection of extrinsic uorescent probe is driven by the consideration of which biological macromolecule or lipid is to be labelled, the requirement for compatibility between the intended uorescent probe (in terms of solubility in water, pH sensitivity and so on) and the properties of the molecule to be labelled Also, choice of the uorescent probe should be consistent with experimental objectives For instance, FRET experiments require that extrinsic donor and acceptor uorophores should be properly matched for their capacity to participate in the FRET effect (see Section 454) One of the rst extrinsic uorophores to be developed was dansyl chloride, which rst emerged in 1951 Dansyl chloride is an amine-reactive probe that is non- uorescent until bound to amine functional groups with the creation of a sulphonamide link (Figures 415 and 417).

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This probe has proven particularly useful in binding interaction experiments since the values of Imax and F are so sensitive to environment (see 7) There is a substantial blue-shift (ca 20 30 nm) and an increase in F as a consequence of the transfer of the probe from a more hydrophilic to a more hydrophobic environment, such as the transfer from bulk aqueous conditions to a hydrophobic binding region in a protein (see Figure 43, to understand the spectral changes which are equivalent) Subsequently, uorescein was developed, which has proved to be a very popular extrinsic uorophore available in several reactive probe forms that all absorb strongly (494 nm, close to the 488 nm wavelength of the argon-ion laser), uoresce strongly and are readily water soluble Direct derivatives of uorescein include tetramethyl rhodamine and Oregon green (Figure 415).

Fluorescein and these corresponding derivatives are a mainstay of many uorescent probe experiments, but they do suffer from signi cant photobleaching, pH-sensitive uorescence, broad emission spectra and uorescence quenching post-bioconjugation In spite of these problems, uorescein and derivatives can give excellent cellular tracking and localisation data in cells, particularly where these extrinsic uorescent probes have been used to label lipids and oligopeptides in conjunction with cyanine dye labelling of DNA (Figure 427) Fluorescein and derivatives, with cyanine dyes, have also proven very useful as matching probes in FRET experiments designed to investigate real time bending, folding and conformational dynamics in isolated biological macromolecule systems, particular those involving proteins and nucleic acids (Figure 428) Fluorescein and derivatives are complemented by the Texas red uorophore, a useful long wavelength extrinsic uorophore (Figure 429).

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