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After you understand the concept, you may discover some new uses for named formulas. One distinct advantage is apparent if you need to modify the formula. You can just change the definition in the Name Box rather than edit each occurrence of the formula.



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a representative marker for cell matrix production RGD coupling, through the enhancement of initial cell density, induced changes in collagen type I production over time as determined by mRNA transcript (monitored via semiquantitative RTPCR) levels at day 7 and protein concentration at day 14 The signi cant increases observed in collagen type I mRNA levels from BMSCs and ACLFs grown on RGDmodi ed matrices are likely the result of an increase in cell density; enhanced cellcell interactions likely induced a switch from cell proliferation to matrix production (differentiation) The increase in collagen type I transcript levels from BMSCs was vefold greater than the increase observed from ACLFs (ie, 180% compared with 30%) ELISA against collagen type I at day 14 con rmed the trends observed in transcription levels at day 7.





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Knobf, M T, 274, 289 Knoll, N, 43, 47, 129, 141 Knopf, D K, 484 Knopf, S, 149, 163 Knopman, D, 492, 506 Knowler, W C, 518, 538 Knudsen, S, 433 Knutson, J F, 447, 461 Kobayashi, J S, 230, 243 Koblin, B A, 221, 243 Koch, G G, 483 Kochanek, K D, 2, 546, 565 Kocsis, J D, 430, 433 Koeller, M, 190 Koepke, D, 451, 460 Ko er, W W, 60, 71 Kogevinas, M, 555, 564 Koh, J, 451, 460 Koh, K B, 81, 92 Kohen, D P, 114, 117 Kohl, H W, 552, 566 Kohler, S, 576, 587 K hler, T, 35, 47 Kohn, R, 529, 540 Kohout, J, 535, 540 Koivula, N, 604, 610 Kojima, H, 213 Kokin, M.

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, 522, 537 Kolak, A M, 384, 387 Kolbe, L J, 557, 563 Kole-Snijders, A M, 295, 315 Kolodny, R C, 204, 213 Kolody, B, 144 Kolonel, L N, 541, 567 Kolterman, O G, 199, 213 Komaroff, A, 367, 368, 369, 371, 372, 375, 385, 386, 387, 388, 389 Komisaruk, B R, 419, 435 Komori, T, 603, 611 Kompier, M A J, 572, 587 Komproe, I H, 65, 71 Kondo, A, 599, 611 Kong, R, 493, 503, 507 Konings, E, 229, 241 Konnarides, C, 206, 210 Konner, M, 128, 140 Konorska, M, 214 Konstantinov, M A, 113, 119 Koob, G F, 149, 152, 162, 168 Koob, J J, 242 Koonin, L M, 543 Koop, C E, 576, 587 Koopman, C, 66, 68 Koopmans, J R.

As in the serial tether, when things are soldered down, you should hook up the wall wart power supply and test the voltages. See the section Step 4: Checking Voltages in 3 for more details, as the process here is almost exactly the same as there. Your standard DC wall wart of around +9V to +24V is used to emulate the Vpwr +16V power coming from the Roomba. The 78L05 voltage regulator will turn that unregulated voltage into the +5VDC needed by the BlueSMiRF and the LED. Using the test points created, hook up the multimeter to Vcc and GND. Connect the wall wart power supply to the Vpwr and GND test points on the circuit. The LED should light up. If it doesn t, disconnect power immediately and check to find out why. Usually it s because the LED is wired backward. If the LED lights, the multimeter should read 5V. When Vpwr is verified, check all the pins of the BlueSMiRF header receptacle. Pay particular attention when testing the voltages in the header receptacle. It should only have +5V going to it, and only on the one header socket meant for +5V.

By 14 days of culture, a 410% increase in BMSC collagen type I production on RGD-modi ed compared with nonmodi ed matrices was observed, supporting the notion of a switch from a proliferative nondifferentiated state at low cell density (eg, 7 days) to a matrix-producing state at high cell densities (14 days) Furthermore, although the effects on collagen type I matrix production may simply be the result of an increase in cell density, the introduction of additional cell binding sites through RGD modi cation may also induce subsequent intracellular signaling events to promote cell matrix production and differentiation [Grzesiak et al, 1997] For tissue engineering, BMSCs should attach rmly on silk matrices, and, appropriately, most BMSCs remained on the RGD-modi ed silk lm after trypsinization for 15 min, whereas most of the cells seeded on tissue culture plastic and EDC/NHS-treated and nonmodi ed silk lms detached.

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