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This free online PDF editor allows you to add and edit texts, images and shapes in your PDF file. Annotate PDF online for free. No registration or installation ... of synthesis of acylglycerols and phospholipids, since these synthetic approaches are important in lipid semi-synthesis should a uorescent tag be required etc. Acylglycerols derive from the controlled esteri cation of hydroxyl functional groups in the alkyltriol known as glycerol. One major problem with the synthesis of pure acylglycerols is that mono- and diacylglycerols easily migrate to adjacent free hydroxyl groups, typically catalysed by acid, base or heat. Therefore, a suitable protecting group strategy is absolutely essential for the chemical synthesis of de ned acylglycerols. For instance, glycerol can react with acetone under acidic conditions to give a 1,2-isopropylidene (acetonide), leaving one primary hydroxyl free for subsequent acylation. Alternatively, benzaldehyde can react to give 1,2- or 1,3-O-benzylidenes that can be separated by crystallisation and used for esteri cation. Typically, acylation can then be performed by carbonyl diimidazole (CDI) activation of the selected fatty acids (Figure 2.16). Thereafter, acetal deprotection can be followed by further esteri cation as appropriate. In the case of phospholipid synthesis, this can be reduced to the controlled esteri cation of l- -glycerophosphocholine.CdCl2 (GPC) made possible by CDI activation of the selected fatty acids (Figure 2.17). Other phospholipids can then be prepared from this phosphatidylcholine by headgroup transphosphatidylation mediated by the enzyme known as phospholipaseD. Lipid synthesis may appear trivial, but there is nothing trivial about the puri cation of lipids to homogeneity post synthesis. In this respect reversed phase HPLC becomes a powerful technique, but using a specially developed evaporative light scattering (ELS) detector to allow for the detection of eluting lipid analytes that do not possess any strong chromophore (see later in 2). word to pdf online: Word to PDF - Convert your DOCX to PDF for Free Online - Zamzar pdf editor software online Delete pages from PDF online ( free ) - PDF Candy
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