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Preview the page in a compatible browser such as Mozilla Firefox, Netscape 6 or better, Safari, and so on to experience the CSS changes (see Figure 14-18).



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Cryovials, 1.8 mL Appropriate carrier (hydroxyapatite/tricalcium phosphate carrier) Procedure (a) Culture the cells with MSC medium until 90% con uent. (b) Wash the dishes three times with PBSA. (c) Add suf cient volume of Trypsin-EDTA solution and incubate at 37 C. (d) Con rm that 80% of cells are detached from the culture surface, then add MSC medium. (e) Centrifuge at 500 g for 6 min. (f) Remove supernate and resuspend cells in MSC medium. (g) Count the cells. (h) Mix 2 4 106 cells suspended in MSC medium with carrier in a 1.8-mL cryovial. (i) Incubate them at 37 C with rotation for 1 h. (j) Transplant them aseptically and subcutaneously into an immunocompromised mouse. We usually use NIH bg-nu/nu-xid mice for transplantation. (k) Harvest them at an appropriate time, and examine histologically. (In the system with HA/TCP carrier and NIH bg-nu/nu-xid mice, a duration of 8 weeks is adequate for the generation of suf cient mineralized matrix by DPSCs and SHED. The time point for harvest depends on the system.)





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When we first exported FishBar to video, it was an experiment We had created the series at 320x240 pixels, which is TV aspect ratio (4:3) So outputting to video was just a matter of scaling up But unfortunately, bitmaps don't scale We had focused so much on getting the file size small for Web delivery, the size of our bitmaps was accordingly pretty tiny, certainly not 720x540 First, we made sure to change the JPG compression for all the bitmaps so that they were not compressed at all Then we exported to video and hoped for the best What we found is that the smoothing option when exporting to video from Flash made up for a lot of the lost quality.

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Also, because we had cut out each of the bitmaps by hand by erasing the backgrounds in Flash with the Eraser tool, the edges of the bitmapped symbols were defined by vectors, and so they had a very clean edge, even after scaling The other thing working in our favor was that the art direction was intentionally rough, so the low-tech look translated well If we had been going for higher production values, we would have had to swap in higher resolution bitmaps and increased the framerate of the animation to achieve a decent result (see the "Swapping in High-Resolution Bitmaps" sidebar) Increasing the framerate would have meant re-animating a lot We had used 22 kHz 16-bit mono sound, which ended up working okay, but it was not broadcast quality.

As we progressed with the FishBar series, we learned a lot of lessons and got much better at going out to video We used 44 kHz 16-bit stereo from the start and no sound compression when going to video We increased the framerate to 15 fps and redid the character art at a higher resolution so we could get better results We still worked at 320x240 in Flash, but used bitmaps that were about 900 pixels high for the characters, scaling them down to fit in the small 320x240 stage For Web delivery, we just compressed the bitmap images like crazy (usually setting JPG compression at a quality of around 15), and because the images were scaled down to fit in the 320x240 stage, the high JPG compression didn't look bad at all.

where Rightm and Leftm take the rightmost and leftmost m bits of w: Rightm (w0 ; w1 ; . . . ; w63 ) (w64 m ; w65 m ; . . . ; w63 ) [ Z m;2 Leftm (w0 ; w1 ; . . . ; w63 ) (w0 ; w1 ; . . . ; wm 1 ) [ Z m;2 is XORed to plaintext to create the ciphertext. When m 64, the output-feedback mode mapping depicted in Fig. 9.7 is a one-toone mapping of Z64,2 onto itself. The average cycle length is 263.

For television delivery, we simply exported the video at 720x540 with no JPG compression, so the bitmaps were then scaled up relative to the Flash document size, but were not bigger than their original import size..

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