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In the gate image, the results are pretty close. All the techniques did well here, and there s not much to choose between them. The Hue/Saturation approach lacks some contrast, so it would probably come down to a choice between the other two. For the portrait, the results are more striking. The Hue/Saturation technique again lacks contrast, while the Channel Mixer version is generally too dark once the skin tones and eyes are boosted. The Black & White approach, though, has a really nice feel, with good contrast and great skin tones. In terms of ease of use, the Hue/Saturation adjustment can t hold a candle to the other two, mainly because you can t see all your color range settings at the same time. This is fiddly to work with. Having to be careful with total color values in the Channel Mixer is also a little tiresome, so although there are more sliders to worry about in the Black & White adjustment, this is the clear winner here. All in all, the new Black & White adjustment probably takes the crown, especially when dealing with skin tones.



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No matter what kind of proposal you re writing, you ll need an organizing framework to get the job done Whether it s 2 or 200 pages, you ll find yourself using most, if not all, of the following proposal categories Executive Summary The most important part of the proposal is this section, which everyone will read especially decision makers The executive summary demonstrates your understanding of the issues and succinctly describes the results the client can expect The summary is not the place to focus on technical descriptions of your approach and methods, but you may describe your strategy and differentiators The summary is likely to be the part of your proposal that is most widely distributed and read So make it clear, concise, jargon-free, and well written Focus squarely on the client s desired results, not the consultant s commercial.





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, 249, 263 Silverstein, J, 193, 213, 474, 483 Silverstein, M D, 116 Silvestri, A, 521, 543 Simard, S, 323, 337 Simeone, C, 358 Simkin, S, 390 Simmens, S J, 239 Simmons, R G, 469, 485 Simms, S, 461 Simon, G E, 323, 324, 337 Simon, R W, 43, 48 Simons, A D, 169, 176, 188 Simons, R N, 323, 335.

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Keep the executive summary to one or two pages, if possible For complex or long proposals, use your judgment, but err on the side of brevity Background Briefly describe why you are being asked to submit a proposal Consultants like this section because they can demonstrate that they understand what the client wants The background statement of one proposal read, After several quarters of improving on-time shipping.

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2. PHYSICAL CHARACTERISTICS Lysins from DNA phages that infect Gram-positive bacteria are generally between 25 and 40 kDa in size except the PlyC for streptococci, which is 114 kDa. This enzyme is unique because it is composed of two separate gene products, PlyCA and PlyCB. Based on biochemical and biophysical studies, the catalytically active PlyC holoenzyme is composed of eight PlyCB subunits for each PlyCA (Nelson et al. 2006). A feature of all other Gram-positive phage lysins is their two-domain structure (Fig. 5.1; Diaz et al. 1990; Garcia et al. 1990). With no exceptions, the N-terminal domain contains the catalytic activity of the enzyme. This activity may be either an endo- -N-acetylglucosaminidase or an N-acetylmuramidase (lysozymes), both of which act on the sugar moiety of the bacterial wall, an endopeptidase that acts on the peptide moiety, or an N-acetylmuramoyl-L-alanine amidase (or amidase), which hydrolyzes the amide bond connecting the glycan strand and peptide moieties (Young 1992; Loessner 2005). Recently, an enzyme with -D-glutaminyl-L-lysine endopeptidase activity has also been reported (Pritchard et al. 2007). In some cases, particularly staphylococcal lysins, two and perhaps even three different catalytic domains may be linked to a single binding domain (Navarre et al. 1999). The C-terminal cell-binding domain (termed the CBD domain), on the other hand, binds to a speci c substrate (usually carbohydrate) found in the cell wall of the host bacterium (Lopez et al. 1992, 1997; Garcia et al. 1998). Ef cient cleavage requires that the binding domain bind to its cell wall substrate, offering some degree of speci city to the enzyme, since these substrates are found only in enzyme-sensitive bacteria. The rst complete crystal structure for the free and cholinebound states of the Cpl-1 lytic enzyme has recently been published (Hermoso et al. 2003). As suspected, the data suggest that choline

The next phase of the uninstall process is the Setup Status window. The Setup Status window keeps you informed on the InstallShield uninstall process. A smaller window appears at the beginning of this process. It is informing you that the device drivers are currently being removed from your system. The removal of the device drivers is the longest portion of the uninstall process. You can halt the uninstall process by clicking the Cancel button.

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