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Cold Harbor Spring Laboratory Press Nichols, J, Zevnik, B, Anastassiadis, K et al (1998) Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factors Oct-4 Cell 95:379 391 Odorico, JS, Kaufman, DS, and Thomson, JA (2001) Multilineage differentiation from human embryonic stem cell lines Stem Cells 19:193 204 Park, JH, Kim, SJ, Oh, EJ, Moon, SY, Roh, SI, Kim, CG, and Yoon, HS (2003) Establishment and maintenance of human embryonic stem cells on STO, a permanently growing cell line Biol Reprod 69(6):2007-14 Pebay, A, Wong, RC, Pitson, SM, Wolvetang, EJ, Peh, GS, Filipczyk, A, Koh, KL, Tellis, I, Nguyen, LT, and Pera, MF (2005).



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Essential roles of sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells Stem Cells 23:1541 1548 Pevny, LH, Sockanathan, S, Placzek, M and Lovell-Badge, R (1998) A role for Sox1 in neural determination Development 131:5512 5525 Pickering, SJ, Braude, PR, Patel, M, Burns, CJ, Trussler, J, Bolton, V and Minger, S (2003) Preimplantation genetic diagnosis as a novel source of embryos for stem cell research Reprod Biomed Online 7(3):353 364 Pickering, SJ, Minger, SL, Patel, M, Taylor, H, Black, C, Burns, CJ, Ekonomou, A, and Braude, PR (2005) Generation of a human embryonic stem cell line encoding the cystic brosis mutation deltaF508, using preimplantation genetic diagnosis Reprod Biomed Online 10:390 397 Pyle, AD, Lock, LF, and Donovan, PJ (2006).





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Neurotrophins mediate human embryonic stem cell survival Nat Biotechnol 24:344 350 Reubinoff, BE, Pera, M, Vajta, G et al (2001) Effective cryopreservation of human embryonic stem cells by the open pulled straw vitri cation method Hum Reprod 16:2187 2194 Reubinoff, BE, Pera, MF, Fong, CY, Trounson, A, and Bongso, A (2000) Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro Nat Biotechnol 18:399 404 Richards, M, Fiong, C-Y, Chan, W-K, Wong, P-C and Bongso, A (2002) Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells Nat Biotechnol 20:933 936 Richards, M, Tan, S, Fong, CY, Biswas, A, Chan, WK, and Bongso, A (2003) Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells Stem Cells 21:546 556.

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Rooney, DE, and Czepulkowski, BH (2001) Human Cytogenetics, A Practical Approach: Constitutional Analysis Oxford University Press, Oxford, UK Rosner, MH, Vigano, MA, Ozato, K et al (1990) A POU-transcription factor in early stem cells and germ cells of the mammalian embryo Nature 345:686 692 Schuldiner, M, Yanuka, O, Itskovitz-Eldor, J, Melton, DA and Benvenisty, N (2000) The effects of eight different growth factors on the differentiation of cells derived from human embryonic stem cells Proc Natl Acad Sci USA 97(21):11307 12..

The template expressions document model is made up of two primary objects: _document and _repeat. The document object contains all the template variables found on the page. For example, if you create an optional region with the name altImageRegion, you can refer to it in a document expression with the following statement:

Divs and AP Elements 101 ......................................................................................................357 Placing <div> Tags ....................................................................................................................360 Defining a CSS rule for a <div> tag ................................................................................360 Inserting the <div> tag ..................................................................................................361 Visualizing <div> tags ....................................................................................................363 Creating AP Elements with Dreamweaver ................................................................................368 Inserting an AP element object ......................................................................................368 Using the Insert Layout Objects AP Div option ......................................................370 Setting default characteristics of an AP element ..............................................................370 Choosing relative instead of absolute positioning ..........................................................372 Modifying an AP Element ........................................................................................................373 Selecting an AP element ................................................................................................373 Resizing an AP element ..................................................................................................374 Moving an AP element ..................................................................................................374

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