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Jam! (Huber, 1999)Jack is a commercially available programming language, which extends the Java language with a number of BDI features (Busetta et al, 2000) The description of the BDI model given here draws upon Bratman et al (1988) and Rao and Georgeff (1992),but is not strictly faithful to either The most obvious difference is that I do not incorporate the notion of the 'filter override' mechanism described in Bratman et al (1988), and I also assume that plans are linear sequences of actions (which is a fairly 'traditional' view of plans), rather than the hierarchically structured collections of goals used by PRS Plans are central to the BDI model of agency.



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Notes: It is essential that the single-cell suspension is free of cell aggregates to enable the passage of a single-cell stream through the ow cytometer, so the cell suspension should be checked on a microscope before use A negative control can also be included to enable background thresholds to be set It is also possible to x cells with a mild solution of paraformaldehyde (02% w/v in PBSA) and keep them at 4 C overnight to preserve samples temporarily should immediate access to the cytometer be restricted 642 Assessment of Developmental Potential 6421 Growth of Teratomas In Vivo It is well known that human embryonic stem cells and their malignant counterparts, human EC stem cells, form complex teratomas consisting of a range of differentiated cell types when engrafted into an immune-de cient host [Andrews et al, 2001; Przyborski 2005; Cooke et al.

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An excellent discussion on the BDI model, focusing in particular on the role of plans in practical reasoning, is Martha Pollack's 1991 Computers and Thought award lecture, presented at the IJCAI-91 conference in Sydney, Australia, and published as 'The Uses of Plans' (Pollack, 1992) Another article, which focuses on the distinction between 'plans as recipes' and 'plans as mental states' is Pollack (1990) It is worth emphasizing that the BDI model is only one solution to the problem of building autonomous rational agents Many other software archtectures for agent systems have been described in the literature (Wooldridge and Jennings, 1995; Brooks, 1999) Other practical reasoning-style architectures include Fischer et al (1996), Jung (1999), Mora el al (1999) and Busetta et al (2000) The BDI model is also interesting because a great deal of effort has been devoted to formalizing it.

In particular, Anand Rao and Michael Georgeff have developed a range of BDI logics, which they use to axiomatize properties of BDI-based practical reasoning agents (Rao and Georgeff, 1991a; Rao et al, 1992; Rao and Georgeff, 1991b; Rao and Georgeff, 1992; Rao and Georgeff, 1993; Rao, 1996b) These models have been extended by others to deal with, for example, communication between agents (Haddadi, 1996)..

, 2006] This was rst demonstrated in by Kleinsmith and Pierce [1964], who showed that the transplantation of a single murine EC cell from one tumor to a new host resulted in the formation of a new teratocarcinoma containing structures similar to those seen in the parent tumor Indeed, this was the way an EC stem cell lineage could be maintained before the derivation of EC stem cell lines Today, many scientists use cell transplantation and the growth of teratomas as a routine method to evaluate the developmental potential of newly derived embryonic stem cell and EC stem cell lines [see for review Przyborski 2005] In vitro experiments enable the characterization of cell differentiation in a more controlled manner, but this approach does have limitations.

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