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Application domains In a .NET managed environment a process can contain several application domains. An application domain is a logically-separate space inside a process. Applications running in different application domains are isolated from each other faults in one application domain cannot affect the code running in other application domains inside the same process. This is mainly achieved by verifying the loaded MSIL code with respect to boundary violations. The code is only executed if no such violations are detected. As a consequence, application domains provide the same isolation features as processes, but with the additional benefit that communication between application domains need not cross process boundaries. Using application domains improves communication performance and scalability, since crossing process boundaries is avoided. However, to communicate between two application domains, .NET Remoting has to be used. Note that application domains are not related to threads. An application domain can contain several threads, and a thread can cross application domains over time. There is a distinct relationship between assemblies and application domains, though. A specific assembly is always executed in one application domain. For example, you can run the same assembly in several application domains, in which case the code is shared, but the data is not.



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The _attrs element is an attr_t type variable indicating which attributes are applied to text (non-space characters) in a window. The bit fields in _attrs are filled according to which attributes are set. See attrset(). _bkgd is a chtype() variable equal to the current background chtype (character and attribute) assigned to the window. This value is set by the bkgd() function.





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had been applied to the azopolymer surface was irradiated with a linear-shaped laser beam of 488-nm wavelength and 10-mW/cm2 optical power density using a cylindrical lens for 5 min, as shown in Fig. 9.8a. The surface was washed in an ultrasonic cleaner and was then observed with an optical microscope, as shown in Fig. 9.8b. Only the microspheres in the linearly irradiated region were strongly immobilized, despite the ultrasonic washing and the relatively large size of the microspheres. DNA molecules were then selected as a potential target material for the immobilization of biological macromolecules. An aqueous solution of 1-mg/mL l-DNA was spotted onto the surface of an azopolymer and covered with a cover glass, where the l-DNA was stained with a uorophore (YOYO-1 iodide, Molecular Probe) in advance, and the surface was then irradiated with the same linearly shaped laser beam for 5 min. The surface was washed for 5 min in an aqueous solution and then observed using a conventional uorescence microscope. Figure 9.8 con rms that the labeled l-DNA was only immobilized in the irradiated region. The same experiment using green uorescent protein (GFP) also indicates the immobilization of protein in the irradiated region. In this way, the authors could demonstrate that an azopolymer can capture micrometer- to nanometer-scaled microobjects, including synthetic polymers and

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biological molecules, on the photoirradiated area The provision of an immobilization process is one of the most essential processing steps that is required to obtain practical biomolecule carriers such as biosensors, bioreactors, or biochips Therefore, a large number of immobilization techniques have already been developed for biological molecules, in which the molecules are immobilized on a carrier using covalent bonds (Feng et al, 2005), ionic bonds (Lee et al, 2003), physical adsorption (Ouyang et al, 2003), cross-linkage of the biomolecules (Levy and Shoseyov, 2004), or microencapsulation (Hartmann, 2005) Chemically induced immobilization methods require optimized processes depending on the structures and properties of the individual biomolecules, which in turn require some complicated procedures (Peluso et al 2003); yet these techniques are widely used Azopolymers can immobilize microobjects that possess a variety of surface characteristics, including negatively charged DNA, charged proteins, and hydrophobic polystyrene.

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