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A complex biochemical pathway can be controlled by photoregulating the activity or availability of a key biomolecule This allows one to turn a biological process on and off at will using light The use inside living organisms is obviously more complicated, but one can reasonably easily apply these principles to control biological processes in industrially relevant settings The ability to quickly and cleanly switch biological activity using a short light pulse may nd application in new micro uidic devices, which need to be able to address speci c device regions and may rely on natural molecular machinery to carry out certain tasks Azobenzenes present unique opportunities in the biological sciences for studying complex biological systems, in addition to controlling them A bacteriorhodopsin analog with a central azobenzene molecule, rather than the retinal, was prepared as a model system for studying rhodopsin (Singh et al, 1996).



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noraw()

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 #include <ncurses.h> int main(void) { int ch; initscr(); raw(); mvaddstr(0,0, Type away, raw mode is on: ); while( getch() != \n ) ; noraw(); mvaddstr(3,0, Type away, raw mode is off: ); while( getch() != \n ) ; endwin(); return 0; }





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E E E Insertion, extraction, and use of INVOCATION CONTEXT information is hidden inside distributed object middleware components, specifically INVOCATION INTERCEPTORS, the REQUESTOR, or the INVOKER. The application logic of clients and remote objects remains independent of INVOCATION CONTEXT information. This works well because in most cases the information in the INVOCATION CONTEXT is only of interest to the add-on service. For situations in which the remote object needs access to INVOCATION CONTEXT data, the distributed object middleware provides a well-defined API. The data in the INVOCATION CONTEXT has to be marshaled just like every other item of information contained in an invocation. As a consequence, the same limitations apply as for operations parameters: the MARSHALLER used must support the data types used. The client- and server-side components that make use of the INVOCATION CONTEXT information must adhere to the same protocol, which means they must agree on a common data structure inside the INVOCATION CONTEXT. Using INVOCATION CONTEXTS, add-on services such as transaction support or security can communicate transparently, that is, without the explicit support of clients and remote objects. The remote objects are kept independent of any contextual data transferred. While INVOCATION CONTEXTS provide a useful and flexible extension mechanism, using them increases the footprint of every remote invocation to which they are added.

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As expected, the azobenzene molecule did not interact as favorably with the protein host as strongly as the natural retinal Despite this, the azo molecules could be coupled into the protein (in the absence of retinal) and led to signi cant shifts in the physicochemical properties of the complex Moreover, the azo molecule could be used as a probe of the inner protein domain (sensing pH, for instance) A particularly elegant experiment involved using azobenzenes to monitor protein folding (Bredenbeck et al, 2003; Sporlein et al, 2002) Femtosecond two-dimensional infrared (2-D IR) spectroscopy was used as a gauge of the distances between carbonyl groups in the peptide An azobenzene chromophore, incorporated inside the polypeptide chain, acted as the photoswitch, initiating a conformational change, hence initiating protein folding, on.

Sample output:

Type away, raw mode is on:

demand. Simultaneous time-resolved measurements of the azo spectra allowed determination of the folding dynamics. This unique measurement of protein-folding behavior was possible because of the phototriggering nature of the azo unit. Ultrafast laser pulse experiments are used to study a large number of chemical reactions, providing detail not possible before. This technique is, however, obviously limited to systems where the chemical events can be phototriggered. By incorporating azobenzene units into new systems, one can generate a phototriggerable system from an otherwise photoinactive one. This strategy can thus be applied to a wide range of problems in chemical dynamics, with biological systems being obvious targets.

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