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Departments of Orthopedic Surgery and Biochemistry1 , Rush University Medical Center, Chicago, Illinois 60612; Department of Bioengineering2 , University of California-San Diego, La Jolla, California 92093-0412 Corresponding author: rsah@ucsdedu 1 2 3 Introduction Culture Conditions for Chondrocytes Preparation of Media and Reagents 31 Media 311 Isolation Medium 312 Culture Medium 32 Saline Wash 33 Enzymes 331 Pronase Digestion Solution 332 Collagenase-P Digestion Solution 333 Papain Enzyme Digestion Solution 334 Pepsin Stock Solution 335 Pancreatic Elastase 34 Alginate Encapsulation 341 Alginate Solution 342 Alginate Dissolving Buffer 343 Calcium Chloride Solution 159 160 160 160 160 160 160 161 161 161 161 162 162 162 162 162 163.



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9. Drag an instance of the button on Stage and then choose Control Test movie. After the movie is published, roll your mouse over the button. The label should start randomly moving around. When you roll off the button, the motion stops. If the motion wasn t frenetic enough for your tastes, edit the jitteryClip and change the value in the timer component s pause for field. Note that the actual object being animated is a symbol. By duplicating the movie clips and button, and then swapping symbols, you can quickly create additional animated buttons.

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Essential Windows CE Application Programming by Robert Burdick Wiley Computer Publishing, John Wiley & Sons, Inc. ISBN: 0471327476 Pub Date: 03/01/99

Object classes are arranged in a hierarchy. Objects can act as containers for other objects. For example, Excel is an object called Application, and it contains other objects, such as Workbook and CommandBar objects. The Workbook object can contain other objects, such as Worksheet objects and Chart objects. A Worksheet object can contain objects such as Range objects, PivotTable objects, and so on. The arrangement of these objects is referred to as Excel s object model. Like objects form a collection. For example, the Worksheets collection consists of all the worksheets in a particular workbook. The CommandBars collection consists of all CommandBar objects. Collections are objects in themselves. When you refer to a contained or member object, you specify its position in the object hierarchy using a period (also known as a dot ) as a separator between the container and the member. For example, you can refer to a workbook named Book1.xls as

Refer to 6 for details concerning the Doppler effect and related considerations with respect to GNSS acquisition of signals.

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where N is the number of states S, M is the number of observations O, A faij g is the matrix of state transition probabilities, B fbij g is the observation symbol probability of symbol Ok in state j, and p is the initial state distribution vector To prepare the HMM for the recognition, a number of steps have to be done: Step 1: Determine a number N of states S The states need not be directly connected with a physical meaning, but it is of great advantage to do so Therefore, M 5 states are chosen, getting the following labels: State S1: open hand State S2: half-open hand State S3: middle position State S4: half-closed hand State S5: closed hand Step 2: Generate a number M of discrete observations O.

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To generate discrete observations, one rst has to deal with the continuous observations, meaning the output cluster centers in Vgrasp and the corresponding joint angles qi It should be mentioned that the clustering process leads to vectors of cluster centers whose elements, although being labelled by a time stamp, are not sorted in increasing order of time Since clustering of several grasps is done independently of each other, the orders of time stamps of the cluster centers are in general different This makes a comparison of test clusters Vgrasp and model clusters Vmodel impossible Therefore, after time clustering has been performed, the output clusters have to be sorted in increasing order of time In the following, cluster centers are assumed to be sorted in that way Next, one has to transform the continuous output cluster centers Vmodel (i), i 1 10 of the model into discrete numbers or labels.

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