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2.2. Smooth Muscle Cell Culture Medium Dulbecco s modi ed Eagle s medium (DMEM) supplemented with 20% fetal bovine serum, penicillin 100 U/ml, and streptomycin 100 g/ml. 2.3. Polyglycolic Acid (PGA) Mesh Surface Treatment Solution Dissolve 40 mg NaOH in 1 L of deionized water to make a 1 N NaOH solution. Make up fresh solution for each PGA treatment. 2.4. Enhanced Culture Medium for Vessel Culture Dulbecco s modi ed Eagle s medium (DMEM) supplemented with 20% fetal bovine serum, penicillin G 100 U/ml, HEPES 5 mM, copper sulfate, 12 nM (CuSO4 5H2 O, 3 ng/ml), L-proline, 0.43 mM (50 g/ml), L-glycine, 0.67 mM (50 g/ml), ascorbic acid, 0.28 mM (50 g/ml), and L-alanine, 0.22 mM (20 g/ml). 2.5. Transmission Electron Microscopy Solutions Fixative: Glutaraldehyde, 0.2 M (2%) in 0.1 M sodium cacodylate buffer. Post xation Solution: Osmium tetroxide, 0.079 M (2%) in 0.1 M sodium cacodylate containing 0.1 M (3.37%) sucrose and 1.2 M calcium chloride at pH 7.2 (300 mOsm). Stain: Uranyl acetate, 0.025 M (1%) in 100% methanol. 2.6. Scanning Electron Microscopy Solutions Fixative: Glutaraldehyde, 0.25 M (2.5%), in 25 mM sodium cacodylate containing 58 mM (2%) sucrose and 1.2 mM calcium chloride at pH 7.2 (300 mOsm). Rinse: 100 mM sodium cacodylate containing 0.1 M (3.37%) sucrose and 1.2 mM calcium chloride at pH 7.2 (300 mOsm). 2.7. Krebs-Henseleit Solution Using deionized water, make a solution consisting of NaCl 118 mM, KCl 4.7 mM, CaCl2 2H2 O 2.5 mM, KH2 PO4 1.2 mM, Mg2 SO4 7H2 O 1.6 mM, sodium pyruvate 2 mM, NaHCO3 24.9 mM, glucose 5.6 mM. Maintain pH of 7.4 7.5 as well as adequate oxygen content of the solution by continuously bubbling with 95%O2 -5% CO2 during use. 3. CULTURE OF TISSUE-ENGINEERED VASCULAR GRAFTS



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This relationship is expressed by the signal-to-noise ratio, which is the difference between the signal level measured in dBmV, and the noise level, also measured in dBmV, both levels being measured at the same point in the system.

If a FreeHand file contains a gradient fill with more than eight colors, Flash creates clipping paths to simulate the appearance of a gradient fill. Clipping paths can increase file size. To minimize file size, use gradient fills with eight colors or fewer in FreeHand.





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COCs and SOA yields for the SAPRC-97 mechanism Reactants CRES+OH ALK1 +OH ALK2 +OH ARO1 +OH ARO2 +OH OLE1 +OH, O3 , NO3 , O OLE2 +OH, O3 , NO3 , O OLE3 +OH, O3 , NO3 , O Description of VOCs Cresols and other alkyl phenols Alkanes with kOH < 104 ppm 1 min 1 Alkanes with kOH > 104 ppm 1 min 1 Aromatics with kOH < 2 104 ppm 1 min 1 , e.g. toluene Aromatics with kOH > 2 104 ppm 1 min 1 , e.g. xylene Alkenes with kOH < 7.5 104 ppm 1 min 1 Alkenes with kOH < 7.5 104 ppm 1 min 1 Monoterpenes, e.g. -pinene and -pinene SOA yielda 1.4 144 402 416 221 9.5 30 762

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rock type (i.e. lithology ), then we talk of lithostratigraphy. Where the subdivision is achieved by means of recognizing distinctive fossils then we talk of biostratigraphy. Where the stratigraphic sequence is constrained by reliable estimates of absolute age of the constituent rocks, we talk about chronostratigraphy. Logically, therefore, we can claim to be pursuing hydrostratigraphy when we subdivide sequences on the basis of the ability of the rocks to store and transmit water. Although this practice has a very long pedigree, having been practised by the Father of Stratigraphy himself, William Smith (1769 1839),v the term hydros(a) Chronostratigraphic units System Neogene Age (Ma) Series Miocene and Pliocene

Figure 7.1 Screenshots of a weather service on different devices: (a) text-only phone; (b) imaging phone or PDA (portrait screen); (c) video-capable phone or PDA; (d) imaging phone or PDA (landscape screen).

Another way to organize your site is by your visitors interests. Remember them We always come back to them eventually and so will you. Can you break your visitors into groups Say, members and nonmembers That s too easy. Here are some other examples: end users, programmers, and other interested parties; doctors and patients; activists and citizens; clients, potential clients, and others. Whatever division you come up with, others will always be the last group in the list. This is simply the nature of the Web. You can t predict who is going to show up on your site.

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