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Figure 7.24. Cell sorting in ow cytometry. (a) The nozzle of the ow cytometer is vibrated axially (dashed ellipse) at a particular frequency by the effect of a drop-forming signal on a piezoelectric crystal. 40 000 vibrations per second gives 40 000 drops per second, (b) Labelled cells are detected by detectors 1 and 2 which pass a signal to a charge generator. This sends a drop-charging signal which confers a change (positive or negative) on the drop containing the labelled cell. In practice, drops in front of and behind that containing the detected cell are also usually charged as well, (c) On leaving the nozzle, charged drops arc de ected between charged plates and collected. Note that a second population of cells in the same sample could be labelled with a negative charge and collected in a second vial.



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1531 Optical Focus Sensors A simple method of determining focus has been implemented in pro ling instruments developed by Brodman and Smilga (1987) and Breitmeier and Ahlers (1987) Illumination from a laser source is focused on the test surface, and the return is split at the optical axis into two parts using a prism Each half of the beam is incident upon a split detector and the difference signal from each split detector is monitored When the focusing lens is too high, the return beam focuses in front of the split detectors and causes a larger signal on the inner detectors; when the lens is too low, the larger signal is on the outer detectors.

The sign of the difference in signal will determine which side of focus the test surface is on, and is used to generate a focus error signal which moves the focusing lens to the correct position When the focusing lens is in the correct position, both the inner and outer detectors have equal signal and the difference signal is zero Two sets of split detectors are used to account for variations in tilt of the test surface A sensor of this type is shown schematically in Figure 1519 (Brodman and Smilga 1987) Because the focus must be adjusted to null the signal at every sampled surface point, this type of pro ler can take a few minutes to generate a three-dimensional surface pro ler The lateral resolution of optical focus sensors is limited by the size of the focus spot at the test surface, usually 10 to 1.

5 mm in diameter The measured surface height at a given sample point will be the average height of the surface over the spot size This means that the smallest measurable features are about 2 mm The measurement area will depend upon the sampling interval and number of data points Another.

Figure 12-6

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