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(x1 Cp and x2 Cq ). If x2 = 0, then A11 x2 = x2 , and so we conclude that s(A11 ). On the other hand, if x2 = 0, then A00 x1 = x1 , so we must have s(A00 ). Thus, s(A) s(A00 ) s(A11 ). Sets s(A) and s(A00 ) s(A11 ) have the same cardinality (i.e., the same number of elements), and so s(A) = s(A00 ) s(A11 ).



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imately 26% of tryptic peptides from the human proteome while covering 96% of total proteins (Table 10.1). As only cysteine-containing peptides are analysed, more proteins can be identi ed in a given analysis time than if all tryptic peptides were analysed. The traditional tag used in this experiment has three components (Figure 10.8). One part contains a thiol-speci c chemical group such as iodoacetic acid/iodoacetamide. This is connected to a linker that can either contain a heavy isotope (e.g. deuterium) or a light isotope (e.g. hydrogen). The third component is biotin which can bind to avidin. Such tags are called isotope-coded af nity tags (ICAT) as the isotope identity allows coding of the two samples (e.g. as test and control) while biotin allows af nity selection for labeled proteins. Samples are exposed to ICATs for long enough to label all SH groups. The two extracts (one now labelled with the light tag and the other with the heavy) are then mixed and digested with trypsin. Cysteine-containing peptides are selected with avidin and analysed by LC-MS-MS Some limitations associated with the traditional tag shown in Figure 10.8 are that heavy/light peptides may not have identical retention on HPLC, some nontagged peptides can bind to avidin nonspeci cally and the biotin moiety makes labelled peptides quite

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There is a lot to be said about taking a strong, proactive approach to network security in today s networks There is a real need ACRONYM ALERT to protect the network from not only indiFDDI Fiber Distributed Data Interface viduals who are out for a challenge, but also to someone who wants to steal or harm data in the network The malicious offender is probably a deeper concern, but either type of security breach can cause harm to the network and to the organization s operation and effectiveness Network security is important regardless of whether you are connected to a public network Without security, your network is vulnerable to an outside (and sometimes even an inside) attack Security precautions need to be taken to protect the data that is being transmitted in the network.

Here is a list of things to consider: Antivirus software is a must for every PC in the network There are too many viruses and worms that can affect not only that end user s node, but also the entire network These attacks can be introduced to the network through e-mail, web sur ng, and even through a software application loaded from a disk to the end user s nodes A rewall is important, as it will help prevent unauthorized access to the network Some services that are offered by rewalls are spoo ng, encryption and decryption, authentication, ltering, and proxy services Not all rewalls will offer all of these, so you will need to determine what suits your needs Maintain a strong network authentication procedure Change passwords often, and control the individuals who can access them Do not discount the importance of physical security.

The default accordion panel, with two tabs, is inserted into the page and the custom Property inspector (see Figure 16-31) displays.

Only authorized individuals should have physical access to network equipment End users need to understand the importance of not leaving their PCs open and available when they are not at their desks Passwords need to be protected Laptops and other mobile gear need to be secure, and users must understand the importance of keeping a good eye on the laptop and the data it contains Utilize network management to keep track of operations within the LAN (more on this in the next section) Again, we discuss network security further in 14..

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