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This allows the synthesis of a tissue equivalent or construct on demand for basic studies on cell-cell and cell-matrix interaction and for in vivo implantation The delity of the construct in terms of its real tissue equivalence naturally depends on identi cation of all the participating cell types in the tissue in vivo and the ability to culture and recombine them in the correct proportions with the correct matrix and juxtaposition So far this has worked best for skin [Michel et al, 1999, Schaller et al, 2002], but even then, melanocytes have only recently been added to the construct, and islet of Langerhans cells are still absent, as are sweat glands and hair follicles, although some progress has been made in this area [Regnier et al, 1997; Laning et al, 1999].

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There are a great many ways in which cells have been recombined to try to simulate tissue, ranging from simply allowing the cells to multilayer by perfusing a monolayer [Kruse et al, 1970] to highly complex perfused membrane (Membroferm [Klement et al, 1987]) or capillary beds [Knazek et al, 1972] These are termed histotypic cultures and aim to attain the density of cells found in the tissue from which the cells were derived (Fig 14) It is possible, using selective media, cloning, or physical separation methods (See Section 34), to isolate puri ed cell strains from disaggregated tissue or primary culture or at rst subculture These puri ed cell populations can then be combined in organotypic culture to recreate both the tissue cell density and, hopefully, the cell interactions and.

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This ontological predisposition can be seen in the primary method used: If a subject could label an appraisal with a word such as threat or good, then these speci c appraisals were assumed to exist and to trigger the emotion Smith and Kirby called for more circumspect inferences from such methods and for the use of methods that focus on the actual processes that constitute appraisals Concluding Comment The ontological approach has been an enormous success indeed, sometimes a victim of its own successes For example, data generated by the FEP unveiled extraordinary complexity within basic emotions Facial, vocal, and instrumental behavior, as well as cognitive appraisal, subjective experience, and physiological changes all show much more variability within each emotion than anticipated (Ortony & Turner, 1990; Smith & Scott, 1997) Further, these separate components do not correlate with each other as highly as anticipated (Lang, 1994).

Figure 5.6 Attacks against location discovery services: (a) masquerade beacon nodes, (b) compromised beacon nodes, and (c) replay attack.

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, & Holmes, R (1985) Mood and pulmonary function in adult asthmatics: A pilot self-monitoring study British Journal of Medical Psychology, 58, 87 94 Stout, C, Kotses, H, & Creer, T L (1997) Improving perception of air ow obstruction in asthma patients Psychosomatic Medicine, 59, 201 206 Taitel, M A, Kotses, H, Bernstein, I L, Bernstein, D I, & Creer, T L (1995) A self-management program for adult asthma Part II: Cost-bene t analysis Journal of Allergy and Clinical Immunology, 95, 672 676 Troisi, R J, Speizer, F E, Willett, W C, Trichopoulos, D, & Rosner, B (1995) Menopause, postmenopausal estrogen preparations, and the risk of adult-onset asthma: A prospective cohort study American Journal of Respiratory and Critical Care Medicine, 152, 1183 188 1 van Peski-Oosterbaan, A S, Spinhoven, P, van der Does, A J W.

management requirements to a managed service provider Unlike the process of selecting a fraud tool or solution, where features and functionality are critical to understanding and evaluating what you are getting, a managed service is supposed to offer the ability to implement and forget meaning that how they do it isn t so much important as how well they do it There isn t just one reason companies decide to consider the use of a managed service in their organization Companies considering managed services for fraud prevention are really asking themselves, Do we want to do this and should we be doing this on our own The fact is all companies will at some point in their lifetime consider the use of a managed service for some part of their fraud-prevention efforts.

Consider the typical motivators for companies to look at managed services: lack of experience, resource constraints, cost, market timing, lack of infrastructure, or lack of will They mirror typical product development build-versus-buy discussions While the motivations may be different from one company to another, the constant similarity with all of these companies is the understanding that fraud is a complex problem and a booming business that will continue to grow during these tough economic times In terms of a strict de nition, managed services describes the process of of oading or outsourcing day-to-day operational management in order to improve, reallocate, or increase ROI within a company s risk management operation A managed service provider (MSP) is a term used to describe third-party vendors that offer managed services Managed services are not the same as hosted software or SaaS.

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