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these disorders are hereditary Hemolytic anemias due to external factors are the immune and microangiopathic hemolytic anemias Certain laboratory features are common to all the hemolytic anemias Haptoglobin, a normal plasma protein that binds and clears hemoglobin released into plasma, may be depressed in hemolytic disorders However, haptoglobin levels are influenced by many factors and, by themselves, are not a reliable indicator of hemolysis When intravascular hemolysis occurs, transient hemoglobinemia occurs Hemoglobin is filtered through the glomerulus and is usually reabsorbed by tubular cells Hemoglobinuria will be present only when the capacity for reabsorption of hemoglobin by these cells is exceeded In its absence, evidence for prior intravascular hemolysis is the presence of hemosiderin in shed renal tubular cells (positive urine hemosiderin) With severe intravascular hemolysis, hemoglobinemia and methemalbuminemia may be present Hemolysis increases the indirect bilirubin, and the total bilirubin may rise to 4 mg/dL Bilirubin levels higher than this may indicate some degree of hepatic dysfunction Serum LDH levels are strikingly elevated in cases of microangiopathic hemolysis (thrombotic thrombocytopenic purpura, hemolytic-uremic syndrome) and may be elevated in other hemolytic anemias



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Hereditary spherocytosis is an autosomal dominant disease of variable severity It is often diagnosed during childhood, but milder cases may be discovered incidentally late in adult life Anemia may or may not be present, since the bone marrow may be able to compensate for shortened red cell survival Severe anemia (aplastic crisis) may occur in folic acid deficiency or when bone marrow compensation is temporarily impaired by infection Chronic hemolysis causes jaundice and pigment (calcium bilirubinate) gallstones, leading to attacks of cholecystitis Examination may reveal icterus and a palpable spleen





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The anemia is of variable severity, and the hematocrit may be normal Reticulocytosis is always present The peripheral blood smear shows the presence of spherocytes, small cells that have lost their central pallor Spherocytes usually make up only a small percentage of red blood cells on the peripheral smear Hereditary spherocytosis is the only important disorder associated with microcytosis and an increased mean corpuscular hemoglobin concentration (MCHC), often greater than 36 g/dL As with other hemolytic disorders, there may be an increase in indirect bilirubin The Coombs test is negative Because spherocytes are red cells that have lost some membrane surface, they are abnormally vulnerable to swelling induced by hypotonic media Increased osmotic fragility merely reflects the presence of spherocytes and does not distinguish hereditary spherocytosis from other spherocytic hemolytic disorders such as autoimmune hemolytic anemia In some laboratories, the osmotic fragility test has been supplanted by ektacytometry, which has the advantages of better reliability and the ability to distinguish spherocytes from other red blood cell abnormalities such as elliptocytosis

Vf Z 44,1 + Z 44,2 + Z 44,0

Positive family history Splenomegaly Spherocytes and increased reticulocytes on peripheral blood smear Microcytic, hyperchromic indices

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system handles all the rest of the work The NIC s driver software is the interconnection between the hardware and the software The NIC driver knows how to communicate with the NIC to send and receive frames, but it can t do anything with the packet Instead, the NIC driver hands the packet off to other programs that know how to deal with all the separate packets and turn them into Web pages, e-mail messages, files, and so forth The Network layer is the last layer that deals directly with hardware All the other layers of the OSI seven-layer model work strictly within software

Hereditary spherocytosis is a disorder of the red blood cell membrane, leading to chronic hemolytic anemia Normally, the red blood cell is a biconcave disk with a diameter of 7 8 mcm The red blood cells must be both strong and deformable strong to withstand the stress of circulating for 120 days and deformable so as to pass through capillaries 3 mcm in diameter and splenic fenestrations in the cords of the red pulp of approximately 2 mcm The red blood cell skeleton, made up primarily of the proteins spectrin and actin, gives the red cells these characteristics of strength and deformability The membrane defect in hereditary spherocytosis is an abnormality in spectrin or actin, the proteins providing most of the scaffolding for the red blood cell membranes The result is a decrease in surface-to-volume ratio that results in a spherical shape of the cell These spherical cells are less deformable and unable to pass through 2-mcm fenestrations in the splenic red pulp Hemolysis takes place because of trapping of red blood cells within the spleen

These patients should receive uninterrupted supplementation with folic acid, 1 mg/d The treatment of choice is splenectomy, which will correct neither the membrane defect nor the spherocytosis but will eliminate the site of hemolysis In very mild cases discovered late in adult life, splenectomy may not be necessary

Kimura F et al Partial splenic embolization for the treatment of hereditary spherocytosis AJR Am J Roentgenol 2003 Oct;181 (4):1021 4 [PMID: 14500222]

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