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syntheses can be used to insert phosphorothioate links into oligo-/polydeoxynucleotides, but a sulphurising agent is needed to replace iodine in order to introduce the non-bridging sulphur atom at each link (Figure 2.9). Should sulphur atoms replace both non-bridging oxygen atoms then achiral phosphorodithioate links are the result. Such links are very stable to hydrolysis in general, but phosphorodithioate links can provoke signi cant deviations from standard oligo-/polynucleotide structures and so are little used in relative terms. In comparison, methylphosphonate linkages that result when a methyl group replaces a non-bridging oxygen atom are undeniably more interesting (Figure 2.10). Unlike the standard phosphodiester links, and the thioate links, methylphosphonates are non-ionic and can therefore help to provide insight into the importance of charge to the structure of oligo-/polynucleotides. Sugar modi cations are easily introduced into oligo-/polydeoxynucleotides or oligo-/ polynucleotides by solid phase synthesis, and these can have many uses, particularly in introducing stability to oligo-/polynucleotide structures. Oligo-/polydeoxynucleotides are comprised of 2 -deoxy- -d-ribofuranose rings that can adopt C2 -endo and C3 -endo conformations ( 1). In contrast, oligo-/polynucleotide -d-ribofuranose rings only exhibit the C3 -endo conformation. Accordingly, 2 -substituents such as uoro, amino, methoxy, alloxy etc stabilise corresponding oligo-/polynucleotides with respect to conformation and also to hydrolytic attack. Most recently, this concept was extended into the development of locked nucleic acids (LNAs), which could be profoundly important in RNA therapeutics in future owing to their conformational and metabolic stability (Figure 2.10). Unsurprisingly, solid phase synthesis also lends itself to the incorporation of non-natural base modi cations into



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This process of taking the average initial monthly fee at each tenure for stopped and active customers is an example of taking a sample average. As discussed in 3, such averages have confidence intervals based on the standard error, so a reasonable enhancement is to include the standard error or confidence bounds in the chart. Figure 7-4 shows the previous chart with 95% confidence bounds for the stopped customers. The confidence bounds for the active customers are so small as to be negligible, so they are not shown on the chart. Because the confidence bounds depend on the number of points at each point, the data is summarized at the weekly level rather than the daily level to narrow the confidence intervals for the stopped customers.





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In the Guides & Grid section of the Preferences dialog box (refer to Figure 8.17), you can change the style and the color of the guides. To open the Guides & Grid section of the Preferences dialog box, choose Edit (Illustrator) Preferences Guides & Grid.

The PHP portion of this photo gallery application is built of three files. The first file is categories.php, which is a static representation of categories being sent to ActionScript. The first part of the code is the category in a multidimensional array format. The category items hold the name, id, and copyright information for each category.

FIGURE 36-4

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is often supported by a GCE. This aggregation is not stressed in any paper in this special issue although it is provided implicitly. Apart from particular features, a GCE usually implies a particular computing model for the Grid, and this model is re ected in the GCE architecture and the view of the Grid presented to the user. For example, object models for applications are very popular, and this object view is re ected in the view of the Grid presented to the user by the GCE. Note the programming model for a GCE is usually the programming of rather large objects one can describe programs and hardware resources as objects without this object model necessarily changing the software model used in applications. With this preamble, we can now classify the papers in this special issue. There are, as always, no absolute classi cations for a complex topic such as distributed Grid systems. Hence it is often the case that these projects can be looked at from many overlapping points of view.

Object Meta Keywords Description Refresh Base Link Description Inserts information that describes or affects the entire document Includes a series of words used by some search engines to index the current Web page and/or site Includes a text description of the current Web page and/or site Reloads the current document or loads a new URL within a specified number of seconds Establishes a reference for all other URLs in the current Web page Inserts a link to an external document, such as a style sheet

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