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2fE -1 What is the power generated by the source in lhe network in Fig. 2PFE- 1

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FIG URE 5-14

Click the Actions tab in the Button Properties dialog box. From the Select Action pull-down menu select Run a JavaScript. Click the Add button and the JavaScript Editor opens. Type the following code in the JavaScript Editor as shown in Figure 28.9.

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Listing 4.3 One-process coding style for a D FF with synchronous enable library ieee; use ieee. std-logic-1164. a l l ; e n t i t y d-ff-en i s port ( clk, reset: in std-logic; en: in std-logic; d : in std-logic; q : out std-logic

13.44 Find the fi nal values of the time fun cti on f( l )

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one S capitis strain was fairly susceptible to the enzyme in vivo in spite of a very high MIC value On the other hand, one methicillin-resistant S epidermidis strain was not susceptible to lysostaphin treatment in vivo despite a relatively low MIC value In a rabbit model of MRSA keratitis, the antibacterial effects of lysostaphin were compared with those of vancomycin (Dajcs et al 2000) Both drugs were administered topically When applied early (4 9 h postinfection), lysostaphin sterilized 100% of the corneas, while vancomycin did not sterilize any Although when administered later (10 15 h postinfection) the enzyme failed to sterilize any cornea, its effectiveness was signi cantly higher (approximately 100,000-fold) than that of vancomycin in terms of reducing colony forming unit per cornea values Importantly, lysostaphin did not exert any deleterious effects on ocular tissues, as graded by the slit-lamp examination.

SDCCH SACCH SACCH SDCCH SDCCH SDCCH R R R R R R R R R R R R R R R R RR R R R R R R R RR 3 2, 0 3, 1 0 1 2 36 TDMA frames 15 TDMA frames

In a separate experiment performed using an S aureus mutant lacking the capability to induce corneal epithelial erosion, lysostaphin was shown to penetrate the intact cornea ef ciently and kill the bacteria As in previous experiments, the decrease in colony forming unit per cornea was signi cantly higher following lysostaphin administration than upon vancomycin treatment An interesting study showed that lysostaphin can also cure ocular infections in immunized animals (Dajcs et al 2002) In this study, rabbits were immunized against lysostaphin by the subcutaneous, intranasal, or intraocular route In separate experiments, the ef cacy of lysostaphin was subsequently evaluated in MRSA keratitis and endophthalmitis In both models, a single topical dose of the enzyme signi cantly decreased bacterial counts in ocular tissues, and in rabbits with endophthalmitis, it was suf cient to completely clear the bacteria, as indicated by sterile vitreous humor in the eyes of the treated animals.

In spite of the generation of neutralizing antibodies, the results obtained in the immunized rabbits were essentially the same as those found in nonimmunized animals Furthermore, no adverse reactions were noted following the administration of lysostaphin to immunized rabbits, and slit-lamp examination (SLE) scores were comparable in both groups of animals These data suggest that lysostaphin could be administered repeatedly in the treatment of ocular infections while maintaining substantial biological activity Another eld of lysostaphin application could be the prophylaxis and treatment of veterinary infections, for example, bovine mastitis Three major ways of preventing and/or treating bovine mastitis have been proposed: (a) topical administration of recombinant lysostaphin, (b) the introduction of the lysostaphin gene into cells of the mammary.

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