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Working together, combine your efforts to make a visual tribute to someone who is leaving (or has left) 10 Draw or make something to commemorate your leaving the _____ 11 On a piece of butcher paper draw or paint a large spiral Along the spiral depict signi cant life events 12 Offer each group member a sheet of butcher paper; have them produce a cartoon strip showing signi cant events in their lives Variation: Write a continuous piece of prose about your life, and select a signi cant image from your writing and draw it..



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so that pan will be set appropriately To get the panning to work correctly, we just need to pass _root.puck1._x for the x argument. It is very convenient to organize your functions in a central place. Here is an example of the Functions Movie Clip used for this game. A Movie Clip can be used as a container for several functions.





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At the mid-point between the extremes of positive and negative interactions is neutralism (listed twice in Figure 8.2, on the left side). Neutralism represents the state in which organisms fail to interact with one another. From a microbial ecology perspective, neutralism is a rare situation that occurs in new, barely colonized habitats (such as recently emerged volcanic islands), or in habitats whose nutrient contents are so low that slow metabolism or dormancy predominates as a lifestyle. Box 8.1 provides information that elaborates upon the eight categories of ecological relationships that extend from symbiosis (also known as mutualism, at the positive extreme) to parasitism (at the negative extreme). Competition, amensalism, and predation are the intermediary categories of negative interaction. Commensalism and synergism (also known as protocooperation) are the intermediary categories of positive interaction. Key aspects of all eight categories include the type of exchange (one way or two way), the speci city of the relationship, and the extent of shared coevolutionary history of the organisms (Figure 8.2). In general, stable, nutrient-rich habitats are the sites where diverse, highly specialized biota are likely to evolve. These stable habitats are also sites where coevolution of symbiotic and parasitic ecological relationships are likely to occur.

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Introduction 208 Preparation of Media and Reagents 209 921 Complete Culture Medium (CCM) 209 922 Trypsin/EDTA 209 923 Crystal Violet Solution 209 924 Bone Differentiation Medium (BDM) 209 925 Alizarin Red S (ARS) Solution 210 926 Fat Differentiation Medium (FDM) 210 927 Oil Red O (ORO) Working Solution 210 928 Stock Solutions (100 ) of BMP2 and -6 210 929 Stock Solution (100 ) of TGF- 3 210 9210 Chondrocyte Differentiation Medium (CDM) 210 Isolation of Human Bone Marrow for MSC Expansion 211 Processing of Human Bone Marrow for MSC Culture 211 Protocol 91 Discontinuous Density Gradient Centrifugation of Human Bone Marrow for MSC Production 211 Expansion and Cryopreservation of MSC Cultures 213 951 Expansion of MSCs from Human Bone Marrow 213 Protocol 92.

Primary Horizontal Axis Title Select None or place a horizontal title below the axis. Primary Vertical Axis Title Select None, Rotated Title, Vertical Title, or Horizontal Title.

Subculture of Mesenchymal Stem Cells (MSCs) 214 952 Cryopreservation and Recovery of MSCs 216 Protocol 93 Cryopreservation of Mesenchymal Stem Cells (MSCs) 216 Protocol 94 Recovery of Cryopreserved Mesenchymal Stem Cells (MSCs) 217 Characterization 218.

Macroscale >1.0 [0.5] Bulk motion Fiber scale 0.005 1.0 [7 10 8 0.5] Laminar and turbulent shear, diffusion Diffusion aided by Microscale <0.005 [7 10 8 ] small scale uid motion

Alkynes are usually much less reactive in halogenation than alkenes 67 Relative reactivities (ka1kenelka1kyne) in the range 102_10 5 were found in comparative studies of chlorination and bromination in acetic acid 15 1,2-Monoaddition products are usually formed except in fluorination, which yields tetrafluoro and solvent incorporated derivatives. In fluorination and chlorination the open ~-halovinyl carbocations were calculated to be more stable than the corresponding cyclic hal onium ions. 34 The formation of nonstereoselective 1,2-dichloroalkenes and regioselective (Markovnikov) solvent incorporated products are in agreement with these calculations. The rate law of bromination of acetylenes is complicated and strongly depends on reaction conditions. 268 Both reaction conditions and alkyne structure affect stereochemistry. Bromination of alkylacetylenes is a stereoselective anti addition process occurring through the cyclic bromonium ion. Phenylacetylenes, in contrast, react according to an AdE 2 mechanism via a vinyl cation intermediate in a nonstereoselective process. Added bromide ions bring about a new, selective bromide ion-catalyzed process. It proceeds through transition state 32 and results in stereoselective anti addition 68 Much higher steroselectivites can be achieved by bromination with CuBr2 69

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