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where si2 represents the sample variance of the observed values of the ith predictor, xi , and Ri2 represents the R 2 value obtained by regressing xi on the other predictor variables. Note that Ri2 will be large when xi is highly correlated with the other predictors. Note that of the two terms in ci , the rst factor, 1/((n 1)si2 ), measures only the intrinsic variability within the of ith predictor, xi . It is the second factor, 1/(1 Ri2 ) that measures the correlation between the ith predictor xi and the remaining predictor variables. For this reason, this second factor is denoted as the variance in ation factor (VIF) for xi : 1 VIFi = 1 Ri2 Can we describe the behavior of the VIF Suppose that xi is completely uncorrelated with the remaining predictors, so that Ri2 = 0. Then we will have VIFi = 1/(1 0) = 1. That is, the minimum value for VIF is 1, which is reached when xi is completely uncorrelated with the remaining predictors. However, as the degree of correlation between xi and the other predictors increases, Ri2 will also increase. In that case, VIFi = 1/(1 Ri2 ) will increase without bound as Ri2 approaches 1. Thus, there is no upper limit to the value that VIFi can take.



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Fig. 3.5 Ranges of hydraulic conductivities encountered in various rock types. The darker the shading, the more common are values in that range for the rocks indicated. Diamict refers to deposits in which large clasts are set within a ner-grained matrix. Carbonates includes limestones and dolostones. Karst refers to classic cave-bearing limestones, and also similarly weathered dolostones and evaporates (especially gypsum). The Basalt lavas referred to here are those which contain lava-tubes. The term plutonic and metamorphic rocks refers to granites, gneiss, schist, etc. The data upon which this gure was based were collated from datasets for all of North America, summarized by Back et al. (1988).

The following explains what the DV operator actually does:

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Chloroform is carcinogenic; wear safety glasses and gloves and always work under a fume hood Read safety data instructions Paper towel Microcentrifuge tubes, 2 ml, with screw cap Microcentrifuge Protocol Note: Contamination with RNA must be minimized Please note: Human skin is the major source of contamination! Precautions to prevent RNase contamination Always wear disposable gloves Change gloves frequently Use sterile RNAse-free, disposable plasticware and automatic pipettes reserved for RNA work RNA is protected from RNase contamination in TRIzol reagent Downstream sample handling requires RNase-free glassware or plasticware Glass and metal items (tweezers, steel balls, beakers) can be exposed to 300 C for 4 h, and plastic items (tubes) can be soaked for 10 min in 01 M NaOH + 1 mM EDTA, rinsed thoroughly with RNAse-free water, and autoclaved RNAse-free containers are also commercially available To prepare RNase-free water, 0.

2. First-order approximation. (The symbols are independent. The frequency of letters matches English text.)

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01% (v/v) diethylpyrocarbonate (DEPC) into UPW in RNase-free glass bottles Incubate for 12 h at room temperature, then autoclave bottle (read instruction for autoclaving liquids) Clean all surfaces (for example, bench top) thoroughly with RNAseZap , wipe with paper towel, rinse with water, and dry with clean paper towel A Scaffolds (a) Grow MSCs on scaffolds in spinner asks according to Protocol 139 (b) Dry scaffolds on a clean paper towel for 3 min on both sides (c) Measure wet weight of the scaffolds Eventually cut the scaffolds into halves and measure the wet weight of halves (d) Transfer each scaffold into a labeled microcentrifuge tube (e) Add 2 steel balls and 1 ml TRIzol per tube (f) Close tube rmly and wrap with Para lm (g) Disintegrate scaffolds by using a MinibeadBeater 6 times at 25,000 rpm for 10 s Place on ice between cycles for cooling.

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