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That is, the difference matrix Rz(h) - Re(h") should be nonnegative-definite for all h. For this reason, we can equivalently interpret (2.8) as the problem of minimizing the error covariance matrix Rj. itself, written as



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For an ideal imager, L should approach A 2. The reduction in L in proportion to the resolving power reduces the optical signal collected by the instrument and requires temporal scanning to achieve SNR and FOV objectives. Coded aperture imaging spectrometers may be constructed using diverse ltering and scanning strategies [86]. For example, the coded aperture design of Section 9.3 actually already functions as 1D spatiospectral imager. Of course, a slit spectrometer is a 1D spatiospectral imager along the y (nondispersed) axis. An independent column code spectrometer, in contrast, images along the x (dispersion) axis. The aligned spectral reconstructions from each column of the aperture code, shown at the center right of Fig. 9.4, correspond to independent spectra for each column of the input aperture. To understand the operation of this instrument, imagine that the power spectral density S(x, l) is uniform as a function of y but varies along the dispersion axis x. Under this assumption, we generalize Eqn. (9.17) to de ne lF 0 0 dx dx0 dl S(x ax j, l nDl )tx (x)hx (x x)px x (10:59) Snj L Equation (9.19) generalizes in turn to gnm X





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The destabilization of the endosomal membrane is thought to be facilitated by the helper lipid [Farhood et al, 1992], enabling the DNA to escape degradation [Zhou X and Huang, 1994; Crystal, 1995] The DNA is thereafter released into the cytoplasm, where it enters the nucleus through the nuclear pores by an unidenti ed mechanism [Seisenberger et al, 2001] Cationic liposomes that are currently used to transfect a variety of cell lines and primary cells are the monocationic cholesterol derivative DC-Chol [Gao and Huang, 1991], the polycationic lipid DMRIE [San et al, 1993], DOTMA (part of Lipofectin ) [Felgner et al, 1987], and DOSPA (part of Lipofectamine ) [Felgner et al, 1995] Transfection of chondrocytes of avian origin has been successful with cationic liposomes like Lipofectin [Long and Linsenmayer, 1995] or Lipofectamine [Pallante et al, 1996].

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However, transfection by these methods with bovine and human articular chondrocytes has not been forthcoming Recently, nonliposomal lipid-mediated methods have been described to offer high transfection ef ciencies in primary chondrocytes Among them, FuGENE 6, a nonliposomal mixture of lipids and other components, has been particularly effective [Madry et al, 2001, 2002, 2003a; Madry and Trippel, 2000; Lefebvre et al, 1998; Dinser et al, 2001] The protocol outlined below will focus on transfection with FuGENE 6..

An RSS feed is simply a text file that contains information about your podcast. RSS feeds are written in Extensible Markup Language (XML), which means all the information is contained within or surrounded by tags. If you ve ever looked at HTML code, the language used to author Web pages, you won t be too frightened by an RSS feed. Here s a simple example of information contained within tags:

n Jitter: The Jitter option works in conjunction with the Gradient option (see the Color box description), so you need to choose a gradient for Jitter to have any effect. The option adds random elements to the spread of colors and opacity levels across the gradient to produce an effect similar to Noise that applies across the whole gradient. The more you drag to the right, the more pronounced the effect becomes. You can also type a percentage value in the box to the right. 0 percent has no effect, while 100 percent gives the most pronounced effect. You can use this option to reduce banding effects when working with indexed-color images, such as GIFs.

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