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Skeletal Muscle Needle Biopsy Protocol 101 Skeletal Muscle Needle Biopsy 4 Isolation and Culture of Human Skeletal Myoblasts Protocol 102 Isolation and Culture of Human Skeletal Myoblasts 5 Characterization of Human Skeletal Myoblasts Protocol 103 Characterization of Human Skeletal Myoblast Cultures 6 Tissue Engineering of Human Skeletal Myoblasts with Clinically Approved Extracellular Matrix Materials Protocol 104 Formation of Silicone Rubber Casting Molds Protocol 105 Tissue-Engineering and Maintenance of Human Skeletal Muscle 7 Applications of Tissue-Engineered Human Skeletal Muscle 71 Gene Therapy 72 Muscle Repair/Replacement Acknowledgments Sources of Materials References 1 INTRODUCTION.

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The objective of a cellular radio operation is to provide a service where mobile subscribers can communicate with any subscriber in the PSTN, where any subscriber in the PSTN can communicate with any mobile subscriber, and where mobile subscribers can communicate among themselves via the cellular radio system. In all cases the service is full duplex. A cellular service company is allotted a radio bandwidth segment to provide this service. Ideally, for full-duplex service, a portion of the bandwidth is assigned for transmission from a cell site to mobile subscriber, and another portion is assigned for transmission from a mobile user to a cell site. Our goal here is to select an access method to provide this service given our bandwidth constraints. We will discuss three generic methods of access: (1) FDMA, (2) TDMA (time division multiple access), and (3) CDMA (code division multiple access). It might be useful for the reader to review our discussion of satellite access in Section 9.3, where we described FMDA and TDMA. However, in this section, the concepts are the same, but some of our constraints and operating parameters are different. It also should be kept in mind that the access technique has an impact on overall cellular bandwidth constraints. TDMA and CDMA are much more ef cient, achieving a considerably greater number of users per unit of RF bandwidth than FDMA.





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Awaits A wait between two transactions that spans sites Can-Wait-For A condition that when satis ed allows one transaction to wait for another local or global transaction Deadlock A state of the DBE in which two or more transactions block each other s process because of locked items Deadlock Avoidance A deadlock handling scheme that uses time as a priority scheme to avoid deadlocks Deadlock Detection A deadlock-handling scheme in which deadlocks are allowed to happen and then are dealt with Deadlock Prevention The most restrictive deadlock handling scheme in which transactions have to secure all the data item locks they need before they are allowed to start Deadly Embrace Another term used for deadlock Distributed Transaction A transaction that leaves its originating site to work on data items that are remote Distributed Wait Die A distributed deadlock-handling scheme that uses the wait die algorithm.

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From these studies, myo brillar proteins or fragments of myo brillar proteins found to change with postmortem storage include actin, myosin heavy chain, titin, myosin light chain I, myosin light chain II, CapZ, co lin, troponin-T, cypher proteins, and myozenin. Sarcoplasmic proteins enolase, phosphoglycerate kinase, pyruvate dehydrogenase, glycogen phosphorylase, triosephosphate isomerase, myokinase, eukaryotic translation initiation factor 5A, -crystallin, creatine kinase, and pyruvate kinase were also found to change with postmortem storage in these studies (Lametsch et al. 2003; Morzel et al. 2004). Using a model in which isolated myo brils were incubated with -calpain under simulated postmortem conditions and protein degradation was measured by combining MALDI-TOF MS with SDS-PAGE and 2DE, one study observed that desmin, actin, myosin heavy chain, myosin light chain I, troponin-T, tropomyosin, thioredoxin, and CapZ were degraded in vitro by -calpain (Lametsch et al. 2004). In both bovine longissimus and semitendinosus muscles, levels of co lin, lactoylglutathione lyase, substrate protein of mitochondrial ATP-dependent proteinase SP-22, HSP27, and HSP20 were found to be different between samples removed at 0 and 24 hours postmortem (Jia et al. 2006). This study also found 15 additional proteins that changed during postmortem storage in either the longissimus or semitendinosus muscles (Jia et al. 2006). In a similar study, thirty-nine proteins were identi ed from bovine longissimus muscle that signi cantly change during the rst 24 hours postmortem (Jia et al. 2007). Proteins undergoing changes included metabolic enzymes, cellular-defense and stressresponse proteins, structural proteins, and proteolytic enzymes. Some of the proteomic changes observed during postmortem aging have been correlated with tenderness measurements. The abundance of actin fragments, myosin heavy

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