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Figure 10.5. Difference gel electrophoresis (DIGE). Two samples (test and control) to be compared are labelled separately with distinct uorescent dyes (e.g. green and red). They are then mixed and separated on a single 2-D SDS PAGE gel. Intensity of spots shows if they are equal in both samples (yellow), up-regulated (green) or down-regulated (red). A colour version of this gure appears in the plate section of this book



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When using the @Entity annotation, it is assumed that you will persist all JavaBean properties of that Java class to the database. For getter/setters of strings, primitives, and various numeric types, you do not have to do anything additional if you want to map to relational database table columns with the same name as the property. In fact, you would have have to explicitly state that you do not want to persist these properties. You can access persistent information by using the entity member variables or JavaBean-style getter/ setter methods on the properties. So there are two ways to define the mapping: field access or property access. Entities can have either persistent fields or persistent properties but not both. In the case of field access all the non-transient member variables are persisted. When you add annotations to an entity s member variables, it is called persistent field annotation. In this case, the persistence implementation accesses entity member variables directly. In the case of property access, all public, protected, and non-transient properties are persisted. When you add annotations to the entity s getter/setter methods for JavaBeans-style properties, it is called persistent properties annotation. In this case, the entity coding must conform to that of JavaBeans. The default type of access for persistent entities is property-level access.





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You may be able to take a course in AutoCAD at a local college or Autodesk Training Center. Many universities and community colleges offer courses in AutoCAD. Such courses may fit your schedule because they re often offered in the evening, over a period of several weeks. Of course, that may not work if you need to get up and running very quickly. I haven t heard of courses on AutoCAD LT, but you can check out your local colleges. However, a course on AutoCAD would certainly help you to learn AutoCAD LT. Autodesk holds a once-a-year conference, called Autodesk University, which offers classes that are taught by top AutoCAD gurus. For more information, go to www.autodesk.com/au. AUGI (www.augi.com) offers one-day seminars around the United States. Autodesk sells short, self-paced courses on both AutoCAD and AutoCAD LT. From the Autodesk home page (www.autodesk.com), click Store, and then click Courseware. If you are a Subscription member, you have access to special courses. For more information about the Subscription program, go to www.autodesk.com and click the Subscription link.

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Density-Based Dry Method The most common density-based dry separation methods include air classi cation discussed above and several technologies drawn from mineral processing and metal recycling industries such as air table classi ers and gravity table separators. Polymer melt density can also be used. Most polymers are incompatible in the solid or liquid state and liquefy at different temperatures [82]. This has resulted in the exploration of a developmental melt centrifuge technique to separate individual plastics from mixtures of plastics. The technique has been applied to the separation of plastics used in several automotive applications [83]. Surface-Property-Based Separation Methods

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