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The hotel s accounting of ce, known as the back of ce, uses the accounting module of a PMS, which assists in the overall nancial management of the hotel (Figure 4-16). PMS simpli es the accounting processes. These include: the labor-intensive posting procedure of accounts payable, which is the amount of money the hotel owes vendors; the transfer of accounts receivable, which is the amount of money owed to the hotel, based



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An unsophisticated approach to solving an instance of a combinatorial NP-complete problem is simply to find all the feasible solutions of a given problem, evaluate their objective functions, and pick the best. However, it is obvious that this approach of complete enumeration is rather inefficient. Although it is possible, in principle, to solve any problem in this way, in practice it is not, because of the huge number of possible solutions to any problem of reasonable size. In case of NP-complete problems, it has been shown that the time required to find exact solutions increases exponentially with the size of the problem [47]. Heuristic methods have been suggested in the literature as an alternative approach to handling such problems.





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A thorough analysis of mineralization and the progression of differentiation relies on biochemical assays and quanti cation of gene expression, respectively Routine assessments involve the quanti cation of total calcium content and the activity of alkaline phosphatase (ALP), a cell surface protein bound to the plasma membrane through phosphatidylinositol phospholipid complexes High ALP activities are associated with active formation of mineralized matrix, and highest levels are found in the mineralization front in bone healing [Bruder and Caplan, 1990] Particularly important in de ning the phenotype of the differentiating stem cells is an understanding of bone tissue development in relation to gene expression of the cells The mRNA expression of the genes encoding important proteins during osteogenic differentiation of hMSC in vitro has not yet been systematically investigated, and results from different studies are often contradictory.

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involvement in sport or physical activity is reflected in their children s level of participation and involvement Most of these studies have focused on the influence of parent behaviors on children s and adolescents level of daily physical activity Operating primarily from a role-modeling perspective, these research studies tested the hypothesis that children s and adolescents level of physical activity would be higher if one or both of their parents were also physically active Mixed support for this parent-child link has been obtained in these studies Some researchers (eg, Anderssen & Wold, 1992; Freedson & Evenson, 1991; Moore et al, 1991; Sallis, Patterson, McKenzie, & Nader, 1988) have found a significant relationship between parents and children s level of physical activity In contrast, other researchers have not observed such a significant parentchild activity link (eg, Brustad, 1993, 1996; Dempsey et al.

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Identi cation of mRNA markers characterizing the progression of hMSC toward the osteogenic lineage is further complicated by the known variability of cells from different individuals or due to differences in the isolation protocols [Phinney et al, 1999] or limitations inherent to the techniques used (ie, semiquantitative techniques like Northern blots or conventional RT-PCR) [Frank et al, 2002] Recently established real-time quantitative RT-PCR technology has made mRNA analysis more reproducible, precise, and sensitive than conventional RT-PCR, because it allows (i) measurement of the amount of ampli ed product with a quantitative laser-based method and (ii) data collection in the early exponential phase of the PCR reaction, when none of the reagents is rate-limiting [Gibson et al, 1996] We developed real-time quantitative RT-PCR assays for genes encoding for (i) osteoblast-related membrane and extracellular matrix molecules (ie.

, 1993; Kimiecik & Horn, 1998; Welk et al, 2003) Some of the inconsistencies in this area may be due to differences across studies in how parents activity level was assessed (eg, through parent self-report or through children s perceptions of their parents activity level) and whether parents activity level was assessed for both mothers and fathers (separately or in a combined index) Furthermore, the age of the child may also be a factor Specifically, several of the studies that did find a significant link between parents and children s level of physical activity (eg, Freedson & Evenson, 1991; Moore et al, 1991; Sallis et al, 1988) were done with relatively younger children (ages 3 to 9 years) Studies where this association was not observed (eg, Dempsey et al, 1993; Kimiecik & Horn, 1998; Welk et al.

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