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periments that removed the centrioles from cells that normally had them demonstrated that the centriole is not necessary for spindle formation. So, although we used to believe that the centriole formed the spindle in many organisms, we now know that the spindle is usually organized around the centrosome, which can function in this capacity without a centriole. The centriole, when present, replicates during the S and G2 phases. When mitosis begins, the centrosome divides and moves to opposite poles of the cell, around the nucleus ( g. 3.10). The centrosomes trail microtubules, forming the spindle, that at this point begin at each centrosome and overlap in the middle of the cell. These are called interpolar microtubules. Microtubules also spread out from the centrosome in the opposite direction from the spindle itself, forming an aster (see g. 3.10). The minus ends of microtubules emanate from the centrosome and the plus ends overlap in the middle of the cell. A third form of tubulin, tubulin, is needed to begin the formation of a microtubule.
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Comments: The T ip- op takes its name from the fact that it toggles between the high and low state Note that the toggling frequency is one half that of the clock Thus the T ip- op also acts as a divide-by-2 counter Counters are explored in more detail in the next subsection
Determine the output of a JK ip- op for the series of inputs given in the table below The initial state of the ip- op is Q0 = 1 J K 0 0 1 1 0 1 1 0 0 0 0 1 1 1
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chromosomes continue to shorten and thicken. The centromeres have already divided, and no new DNA synthesis is needed for the process to be completed. At this point, the sister chromatids are kept together by a complex, called cohesin, made up of at least four different protiens. Spindle bers are initially nucleated at the centrosome and grow outward into the cytoplasm ( g. 3.12). Some of these bers capture a kinetochore, the proteinaceous complex at the centromere of each sister chromatid; these bers are called kinetochore microtubules. At rst, one kinetochore or the other randomly attaches to a spindle ber. As the microtubules further move the chromosomes and as new microtubules attach and old microtubules break, each sister kinetochore eventually attaches to microtubules emanating from different poles. This ensures that sister chromatids move to opposite poles during anaphase. The number of microtubules that attach to each kinetochore differs in different species. It seems that 1 attaches to each kinetochore in yeast, 4 to 7 attach to each kinetochore in the cells of a rat fetus, and 70 to 150 attach in the plant Haemanthus (see g. 3.12).
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Mt Palomar telescope in the US that weighs 1,000,000 lbs and moves at a speed equal to that of the rotating Earth is supported by externally pressurized bearings and uses only a 1/12 hp motor (Figure 649) Figure 649 shows the 102 in diameter hydrostatic journal bearing that is used in large-size cascade mills The application of hydrostatic bearings can also be traced to marine engines and radar systems In marine engines, the main and big end bearings consist of indium-plated lead-bronze lined, steel shells These bearings are Fig 650: A finished 102 in diameter hydrostatic journal precision manufactured and are ready bearing mounted in its support pedestal [20] for fitting The engines are equipped with a force-feed lubrication system with oil filters of the full-flow type and an oil cooler The AN/FPS-24 search radar that serves as the backbone of the United States air defense uses hydrostatic bearings to support its 85 tonne antenna (Figure 651) Goodyear Aerospace developed the bearings
Known Quantities: JK ip- op truth table (Figure 148) Find: Output of RS ip- op, Q, as a function of the input transitions
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